SPECIES OF STREPTOMYCES 53 



Glucose broth: Surface ring cream-colored to brown. Aerial mycelium 

 powdery white. Soluble pigment slight, reddish brown. 



Nitrate: Reduction positive. 



Antagonistic properties: Produces griseolutein, a yellow antibiotic active 

 against gram-positive and gram-negative bacteria. 



27. Streptomyces bobiliae (Waksman and Curtis) Waksman and Hen- 

 rici. (Waksman, S. A., and Curtis, R. E., Soil Sci., 1, 1916, 121.) 



Aerial mycelium: Few close spirals of a dextrorse type. 



Synthetic agar: Growth abundant, glossy, wrinkled, elevated, coral-red 

 becoming deep red. Aerial mycelium scant, white. 



Nutrient agar: Growth restricted, glossy, gray, becoming brownish. 



Potato: Growth thin, yellowish, becoming red, dry, and wrinkled. 



Gelatin: Surface growth dense, cream-colored to brownish. Liquefaction 

 rapid. 



Milk: Ring dark brown. No coagulation. Peptonization. 



Starch: Growth restricted, finely wrinkled, coral-red with hyaline mar- 

 gin. Hydrolysis. 



Glucose broth: Round colonies in fluid. Flaky sediment. 



Nitrate: Reduction to nitrites. 



Temperature: Optimum 37°C. 



Antagonistic properties: Positive. 



Remarks: Soluble brown pigment formed. 



28. Streptomyces aurantiacus (Gasperini emend. Krassilnikov) comb, 

 nov. (Krassilnikov, N. A., Actinomycetales, Akad. Nauk. USSR, Moskau, 

 1941, 36.) 



Vegetative growth: Lichnoid, dry, compact. Colored bright orange or 

 golden; color does not change into red or yellow on continued incubation. 

 Pigment insoluble in medium, but soluble in organic solvents. Produces an 

 abundance of chlamydospores. 



Aerial mycelium : Poorly developed or completely absent on many media ; 

 nonseptated. Sporophores form spirals with 3-5 turns. Spores spherical to 

 oval, 0.7-0.9 by 0.6-0.8 p. 



Potato: Soluble pigment brown. 



Gelatin: Liquefaction slow, after 20-30 days' incubation. 



Milk: Peptonization weak, usually without previous coagulation. 



Starch: Hydrolysis slow. 



Cellulose: No growth. 



Nitrate: No reduction. 



Sucrose: No inversion. 



