56 ACTINOMYCETES 



Gelatin: Growth flocculent, through medium. Liquefaction slow. No 

 soluble pigment. 



Milk: Ring cream-colored. No aerial mycelium. Peptonization very rapid. 



Starch: Growth very limited, similar to that on synthetic agar. Hy- 

 drolysis. 



Nutrient broth: Surface growth in clumps, cream-colored. Aerial myce- 

 lium gray. 



33. Streptomyces diastaticus (Krainsky) Waksman and Henrici- 

 (Krainsky, A., Centrbl. Bakt. II, 41, 1914, 639-688.) 



Aerial mycelium: Filaments may show fine, long, narrow spirals. Conidia 

 oval, 1.0-1.2 by 1.1-1.5 p. 



Synthetic agar: Growth thin, gray, spreading. Aerial mycelium white, 

 becoming drab-gray. 



Nutrient agar: Growth cream-colored. Aerial mycelium thin. 



Glucose agar: Growth yellowish, spreading. No aerial mycelium. 



Potato: Growth abundant, wrinkled, cream-colored with greenish tinge. 



Gelatin: Liquefaction; with small, cream-colored flakes in liquid. 



Milk: Ring brownish; coagulation; peptonization in 25 to 30 days, be- 

 coming faintly alkaline. 



Starch: Growth thin, colorless, spreading. Aerial mycelium gray. Hy- 

 drolysis. 



Glucose broth: Ring gray, with grayish colonies in bottom of tube. 



Nitrate: Reduction to nitrite. 



Temperature: Optimum 37°C. 



Antagonistic properties: Limited. 



Remarks: Brown to dark brown soluble pigment formed. 



34. Streptomyces canescus Hickey et al. (Hickey, R. J., Corum, C. J., 

 Hidy, P. H., Cohen, I. R., Nager, U. F. B., and Kropp, E., Antibiotics 

 & Chemotherapy, 2, 1952, 472-483.) 



Aerial mycelium: At first white, becoming, on sporulation, gray-white 

 to gray. Conidiophores straight or curved, not forming any spirals, richly 

 septate. Spores globose, 1.0-1.3 by 1.3-2.6 m- 



Potato: Growth light gray, spreading, wrinkled. Deep brown pigment 

 diffused throughout. 



Gelatin: Liquefaction rapid. 



Milk: At 36°C becomes alkaline (pH 8.4). Soft, rennet curd formed 

 after 48 hours is completely peptonized in 12 days. 



Starch: Hydrolysis strong. 



Nitrate: No reduction. 



