SPECIES OF STREPTOMYCES 57 



Egg medium : Surf ace growth tan, very wrinkled. No sporulation observed 

 after 10 days' incubation; small amount of white sporulation observed in 

 14 days. Soluble brown pigment formed. After 21 days, odor of hydrogen 

 sulfide detected. Liquefaction after 28 days very slow. 



Bennett's agar: Colonies circular, effuse to convex, edge filamentous; 

 powdery aerial mycelium; varying from gray-white to gray; reverse 

 brown. No diffusible pigment. 



Sabouraud's agar: Growth first white, dull-shiny, spreading, translucent; 

 reverse tan. After 7 days' incubation, growth beaded, slightly wrinkled at 

 base of the slant, grayish white; reverse tan to amber. Amber pigment 

 diffused throughout medium. After 14 days aerial mycelium faintly green- 

 ish. 



Calcium malate agar: Mycelium gray to rose-gray; reverse yellow to tan; 

 digestion of calcium malate slight at edge of colony. No soluble pigment. 



Temperature: Optimum 36°C. 



Antagonistic properties: Produces ascosin. 



Source: Contaminated fungus plate. 



Remarks: The organism utilizes the following as sole carbon sources: 

 dextrose, arabinose, trehalose, xylose, sucrose, maltose, galactose, dextrin, 

 soluble starch, mannitol, glycerine, and salicin. No growth observed with 

 sorbose, melizatose, dulcitol, rhamnose, sorbitol, melibiose, phenol, raffi- 

 nose, and lactose. 



35. Streptomyces fimicarius (Duch6) Waksman and Henrici. (Duch6, 

 J., Les actinomyces du groupe albus, P. Lechevalier, Paris, 1934.) 



Synthetic agar: Growth yellowish masses, with yellowish white aerial 

 mycelium; reverse orange-colored; faint yellowish soluble pigment. 



Nutrient agar: Growth cream-colored with white aerial mycelium; re- 

 verse yellowish. 



Potato: Growth cream-colored to yellowish with whitish aerial mycelium; 

 reddish-brown pigmentation. 



Gelatin: Punctiform colonies with whitish aerial mycelium; soluble pig- 

 ment reddish. Liquefaction. 



Milk: Growth colorless, becoming covered with whitish aerial mycelium; 

 slow peptonization of milk, which becomes rose-colored, finally changing 

 to brownish red. 



Asparagine agar: Growth cream-colored with whitish aerial mycelium; 

 reverse cream-colored to slight ocher. 



Asparagine solution: Vegetative filaments 0.5-0.6 m long; branching 

 aerial mycelium 0.8-1.0 /t, forming numerous conidia; flaky growth pro- 

 duced on bottom; surface growth becomes covered with a white aerial 

 mycelium; reverse brownish red. 



