60 ACTINOMYCETES 



Occasionally pomegranate-purple soluble pigment is formed. 



Potato: Good folded growth, with chalky white aerial mycelium. At 

 35°-36°C, a reddish purple pigment is formed. 



Gelatin: Rapid liquefaction. 



Milk: Coagulation, followed by peptonization. 



Starch agar: Good growth, in form of discrete colonies with white aerial 

 mycelium in center and periphery colorless and embedded. Hydrolysis. 



Cellulose: Poor growth. 



Nitrate: Traces of nitrite produced. 



Glucose-asparagine agar : Wrinkled tan growth with gray and white knob- 

 like projections. Reverse of growth dark gray. At 35°-36°C, scant, shell- 

 pink aerial mycelium; limited shell-pink diffusible pigment. 



Blood agar: Good growth, consisting of convex, lobate colonies, with 

 central perforation. Heavy, chalky white aerial mycelium. No hemolysis, 

 but darkening of blood. 



Honey broth: Heavy, white ring on surface; flocculent sediment. Broth 

 clear. 



Antagonistic properties: Produces an antifungal agent, active against 

 various yeast-like and filamentous fungi, nystatin, and an antibacterial 

 agent, phalamycin. 



39. Streptomyces roseochromogenus (Krainsky, emend. Jensen) Waks- 

 man and Henrici. (Jensen, H. L., Proc. Linnean Soc. N. S. Wales, 56, 1931, 

 359.) 



Vegetative growth: Pigmented red to rose-colored; produces dark brown 

 soluble substance. 



Aerial mycelium: Well developed. Sporophores produce numerous open 

 and closed spirals. Spores 1.0-1.2 by 1.5-3.0 m- 



Synthetic agar: Growth thin, spreading, colorless. Aerial mycelium thin, 

 pale, brownish. 



Nutrient agar: Growth white, becoming yellowish. 



Potato: Growth much- wrinkled, brownish. 



Gelatin: Small cream-colored colonies in bottom of liquid. Soluble brown 

 pigment. Liquefaction medium. 



Milk: Coagulation limited; peptonization slow. 



Starch: Growth colorless, spreading; hydrolysis good. 



Glucose broth: Ring cream-colored, with flaky sediment. 



Nitrate: Reduction to nitrite. 



Antagonistic properties: Active against various bacteria; produces an 

 antibiotic roseomycin. 



40. Streptomyces cinnamonensis Okami, Madeda, Kosaka, Taya, 

 and Umezawa. (Okami, Y., Madeda, K., Kosaka, H., Taya, 0., and 

 Umezawa, H., Japan. Med. J., 6 y 1953.) 



