SPECIES OF STIIEPTOMYCES 61 



Vegetative growth : Fine branching mycelium. 



Aerial mycelium: Diameter 0.8-1.2 /z; long filamentous mycelium; no 

 spirals. Spores elliptical to oval. 



Synthetic glycerol agar: Growth colorless, spreading; scant white aerial 

 mycelium or white with pale cinnamon-pinkish (Rdg. XXIX) to light 

 brownish vinaceous tinge (Rdg. XXXIX) ; no soluble pigment. 



Nutrient agar: Growth colorless to dark; no aerial mycelium; none or 

 slightly brown soluble pigment. 



Potato: Growth light cream to dark-colored; no aerial mycelium. No 

 soluble pigment; later black pigment produced around growth. 



Loeffler's serum media: Growth colorless to dark-colored; no aerial my- 

 celium; soluble brown pigment; no liquefaction. 



Gelatin: Growth colorless to dark brownish; aerial mycelium in form of 

 white patches ; soluble pigment brown ; liquefaction none or very slow. 



Milk: Growth cream-colored to brownish surface ring; no aerial myce- 

 lium or scant white; no soluble pigment or slightly brown; no coagulation; 

 peptonization none or very slow. 



Starch agar: Growth colorless, spreading; aerial mycelium white with 

 pinkish tinge; no soluble pigment; good hydrolysis. 



Cellulose: No decomposition. 



Nitrate: No reduction. 



Glucose-asparagme agar: Growth colorless to light cream-colored; white 

 to white-pinkish cinnamon aerial mycelium to light brownish vinaceous 

 tinge. No soluble pigment. 



Blood agar: Growth dark to brownish; no aerial mycelium; soluble brown 

 pigment; no hemolysis. 



Antagonistic properties: Produces an antibiotic active against myco- 

 bacteria identical with actithiazic acid or thiozolidone. 



Remarks: The culture resembles S. roseochromogenus, in the color of the 

 growth, and in points of none or slow liquefaction of coagulated serum and 

 gelatin. It differs in that S. roseochromogenus forms numerous open spirals 

 and produces nitrite from nitrate. 



The following carbon sources were utilized: sucrose, mannose, dextrin, 

 galactose, glycerol, fructose, glucose, maltose, mannitol, xylose, and so- 

 dium succinate. Arabinose, esculin, rhamnose, dulcitol, sodium acetate, 

 inulin, and lactose were not utilized. Salicin and raffinose were negative. 



41. Streptomyces olivochromogenus (Bergey et el.) Waksman and 

 Henrici. (Waksman, S. A., Soil Sci., 8, 1919, 106.) 



Aerial mycelium: Filaments with numerous close spirals. Conidia oval or 

 elliptical. 



Synthetic agar: Growth white, spreading. Aerial mycelium ash-gray with 

 brownish tinge. 



