62 ACTINOMYCETES 



Nutrient agar: Growth wrinkled, brown, becoming gray-green. 

 Glucose agar: Growth abundant, natal-brown to almost black, entire 

 margin. 



Potato: Colonies small, wrinkled, black. 



Gelatin: Surface growth cream-colored, spreading. Liquefaction rapid. 



Milk: Ring dark brown; coagulation; peptonization, becoming alkaline. 



Starch: Growth transparent, spreading. Hydrolysis. 



Glucose broth: Growth thin, brown; flaky sediment. 



Nitrate: Reduction to nitrite faint. 



Temperature: Optimum 37°C. 



Antagonistic properties: Positive. 



Remarks: Soluble brown pigment formed. 



42. Streptomyces resistomycificus Lindenbein (Lindenbein, W., Arch. 

 Mikrobiol., 17, 1952, 361-383.) 



Aerial mycelium: Hyphae long with curling tips. Spores short, oval. Pig- 

 ment of mycelium becomes pink to pink-gray. 



Synthetic agar: Growth good, crumb-like, dark brown. Aerial mycelium 

 velvety chalk-white, later ash-gray. Soluble pigment red-brown. 



Nutrient agar: Growth good, crumb-like. Aerial mycelium powdery blue- 

 gray. Soluble pigment dark brown. 



Glucose agar: Growth crumb-like with dark brown reverse. Aerial myce- 

 lim velvety chalk-white. Soluble pigment reddish to dark brown. 



Potato: Growth good, brownish black. Aerial myeclium powdery, red- 

 dish white. Soluble pigment dark brown. 



Gelatin: Growth good, dark brown. Aerial mycelium powdery white- 

 gray. Soluble pigment chestnut-brown. Liquefaction good. 



Milk: Growth good, lichnoid; dark brown reverse. Aerial mycelium vel- 

 vety chalk-white, later yellowish red. Soluble pigment dark brown. Pep- 

 tonization none or slight. 



Starch: Growth good, lichnoid; reddish brown reverse. Aerial mycelium 

 velvety gray-white, later becoming red-gray. Soluble pigment lacking or 

 very equally reddish brown. Hydrolysis strong. 



Cellulose: No growth. 



Glucose broth: Colonies large, floating, light yellow. Aerial mycelium 

 cottony white to red. Soluble pigment light yellow. 



Glucose-asparagine agar : Growth crumb-like ; light brown reverse. Aerial 

 mycelium velvety ash-gray. Soluble pigment yellow-brown. 



Calcium malate agar: Growth strong, crumb-like; dark brown reverse. 

 Aerial mycelium velvety ash-gray. Soluble pigment ash-gray. 



Antagonistic properties: Produces resistomycin, which is present in the 

 mycelium and which is active against gram-positive bacteria. 



