70 ACTINOMYCETES 



Synthetic agar: Growth colorless, becoming brown to almost black. Aerial 

 mycelium abundant, white with brownish shade. 



Nutrient agar: Growth thin, cream-colored, becoming gray. 



Glucose agar: Growth restricted, much-folded, brown. 



Potato: Growth brown to almost black. 



Gelatin: Surface growth abundant, spreading, cream-colored, becoming 

 brown. Liquefaction slow. 



Milk: Ring dark, almost black; coagulation, with slow peptonization, 

 faintly alkaline reaction. 



Starch: Growth spreading, brownish, becoming brown. Hydrolysis. 



Glucose broth: Pellicle dense, wrinkled. 



Nitrate: Reduction to nitrite. 



Temperature: Optimum 25°C. 



Antagonistic properties: Strong. 



Remarks: Soluble brown pigment formed. 



54. Streptomyces aureus (Waksman and Curtis) Waksman and Hen- 

 rici. (Waksman, S. A., and Curtis, R. E., Soil Sci., 1, 1916, 124.) 



Aerial mycelium: Shows numerous spirals. Conidia spherical to oval, 

 0.6-1.0 by 0.8-1.4 /*. 



Synthetic agar: Growth thin, spreading, colorless. Aerial mycelium thin, 

 powdery, gray, becoming cinnamon-drab. 



Nutrient agar : Growth restricted, gray. 



Glucose agar: Growth spreading, light orange, raised center, hyaline 

 margin. 



Potato: Growth abundant, wrinkled, brown, becoming black. 



Gelatin: Fair, cream-colored surface growth, becoming brown, spreading. 

 Liquefied. 



Milk: Ring black. No coagulation. Peptonization doubtful. 



Starch: Growth thin, transparent, spreading. Hydrolysis positive. 



Glucose broth: Ring thin, brownish; flaky sediment. 



Nitrate: Reduction to nitrite. 



Temperature: Optimum 25°C. 



Antagonistic properties: Produces fungicidin RAW (antimycoin), a sub- 

 stance active against various fungi. Some strains produce luteomycin. 



Remarks: Soluble brown pigment formed in organic media. 



55. Streptomyces tanashiensis Hata, Ohki, and Higuchi (Hata, T., 

 Ohki, N., and Higuchi, T., J. Antibiotics [Japan] 5, 1952, 529-534, 313-325.) 



Aerial mycelium: Hyphae almost straight; lightly open spirals; spores 

 spherical to oval, 1.0 by 1.2 ju. 



Synthetic agar: Growth grayish yellow. Aerial mycelium white-gray turn- 

 ing brownish gray. Soluble pigment light yellow. 



