SPECIES OF STREPTOMi'CES 89 



Glucose agar: Aerial mycelium in about 12 days, rose-yellow. 



Potato: Growth yellowish. No aerial mycelium. 



Gelatin: Colonies small, yellowish. Liquefaction rapid. 



Milk: Coagulation and peptonization rapid. 



Starch: Diastatic action strong. 



Cellulose: Growth scant. 



Glucose broth: Colonies in depth small, spherical. 



Nitrate: Reduction to nitrite. 



Calcium-malate agar: Colonies minute, yellow. No aerial mycelium. 



Temperature: Optimum 25°C. 



Antagonistic properties: Positive. 



87. Streptomyces cacaoi (Waksman) Waksman and Henrici. (Waks- 

 man, in Bunting, Ann. Appl. Biol., 19, 1932, 515.) 



Aerial mycelium: Long with considerable spiral formation; spirals long 

 and open, not compact. 



Synthetic agar: Growth thin, yellowish, later turning reddish brown; no 

 soluble pigment; aerial mycelium light gray to mouse-gray, with white 

 edge. Typical odor of streptomyces. 



Nutrient agar: Growth brown-colored, covered with tiny patches of 

 ivory-colored aerial mycelium. 



Glucose agar: Same as on synthetic agar. 



Potato: Growth abundant, brownish; aerial mycelium white to mouse- 

 gray. 



Gelatin: Growth flocculent. No aerial mycelium. Liquefaction rapid. No 

 pigment production. 



Nitrate: Reduction limited. 



Antagonistic properties : Certain strains produce an antibiotic designated 

 as cacaomycetin. 



Source: Three strains isolated from cacao beans in Nigeria. There were 

 slight differences among the three strains; the foregoing description is of 

 Strain I. 



Remarks: Strong proteolytic enzymes acting on casein; strong diastatic 

 action, no sugar or dextrin left in 1 per cent starch solution after a few days. 



88. Streptomyces novaecaesareae (Waksman and Curtis) Waksman 

 and Henrici. (Waksman, S. A., and Curtis, R. E., Soil Sci., 1, 1916, 111.) 



Aerial mycelium: Filaments with both straight and spiral aerial hyphae; 

 spirals dextrorse. Conidia oval to elongate. 



Synthetic agar: Growth gray, becoming bluish, glossy, much-wrinkled. 

 Aerial mycelium appears late; white. 



Nutrient agar: Growth thin, cream-colored. 



