114 ACTINOMYCETES 



Milk: Growth very slight. No coagulation; no peptonization. 

 Starch: Hydrolysis. 



Glucose broth: Numerous minute, whitish colonies at bottom of con- 

 tainer. 

 Nitrate: No reduction. 



Glycerol synthetic solution: Poor flaky growth at bottom. 

 Tyrosinase reaction : Negative. 

 Temperature: Grows well at 37.5°C. 

 Anerobic growth: Good. 



131. Streptomyces coroniformis (Millard and Burr) comb. nov. (Mil- 

 lard, W. A., and Burr, S., Ann. Appl. Biol., 13, 1926, 580.) 



Aerial mycelium: Simple branching. Spores oval, 0.8 by 0.6 y.. 



Synthetic sucrose agar: Growth in form of discrete colonies partially 

 coalescing, gray to greenish. White aerial mycelium covering edges 

 of growth. 



Synthetic glucose agar : Growth in form of discrete colonies growing into 

 the medium, poor grayish. No aerial mycelium. 



Nutrient potato agar: Growth wrinkled, grayish. No aerial mycelium. 



Potato: Growth raised, grayish. Aerial mycelium white. Plug pigmented 

 brownish around and under growth. 



Gelatin: Growth fair. Liquefaction slow or none. 



Milk: A few colonies on surface. No coagulation. Peptonization limited. 



Starch: Hydrolysis negative to trace. 



Glucose broth: Fair growth at bottom. 



Nitrate: Reduction limited. 



Glycerol synthetic solution : Very poor growth in form of small flakes at 

 bottom. 



Tyrosinase reaction : Negative. 



Temperature: Growth fair at 37.5°C. 



Habitat: Potato scab. 



132. Streptomyces spiralis (Millard and Burr) comb. nov. (Millard, 

 W. A., and Burr, S., Ann. Appl. Biol., IS, 1926, 580.) 



Vegetative growth: Lichnoid, smooth, yellowish golden. No soluble pig- 

 ment. 



Aerial mycelium: Sporophores straight or spiral-forming. Spores cylindri- 

 cal, 1.0-1.7 by 0.9 m. 



Synthetic sucrose agar: Growth rough or granular; aerial mycelium vi- 

 naceous-buff to dark grayish olive. Soluble pigment pale vinaceous-fawn- 

 colored. 



