SPECIES OF STREPTOMYCES 119 



Vegetative growth: Wavy mycelium branched in a monopodial form, 1/x 

 in diameter. On agar, two types of colonies are produced — one small, flat, 

 regular, white; the other large, thick, irregular, yellowish. 



Aerial mycelium: Producing spirals; conidia oval, 0.8-1.5 by 0.8 /z- 



Synthetic agar: Colonies small; aerial mycelium powdery, white. No 

 soluble pigment. 



Nutrient agar: Growth poor, thin, yellowish; aerial mycelium powdery, 

 white. Soluble pigment brown. 



Peptone agar: Growth limited, cream-colored; aerial mycelium powdery, 

 white. Soluble pigment very slight, brown-reddish. 



Gelatin: Growth poor, flaky, white; liquefaction limited. 



Milk: Growth slow; aerial mycelium white. No soluble pigment. At 

 25°C no coagulation; at 37°C coagulation after 20 days. No peptonization. 

 No change in reaction. 



Starch: Colonies thin; aerial mycelium powdery, white. No soluble pig- 

 ment. 



Nitrate: Reduction. 



Glucose-peptone medium: Numerous colonies covering surface. Soluble 

 pigment slight, brownish. 



Glycerol potato: Orange-reddish punctiform colonies growing together. A 

 thick crust. Limited white aerial mycelium appearing very slowly. No 

 soluble pigment. 



Proteolysis: No action on coagulated serum. 



Source: Dog septicemia (thoracic, abdominal, and brain lesions). 



Remarks: Pathogenic for guinea pig and rabbit. Not pathogenic for dog 

 after laboratory growth. 



140. Streptomyces upcottii (Erikson) Waksman and Henrici. (Gib- 

 son, A. G., J. Pathol., and Bacterid., 28, 1920, 357; Erikson, D., Med. 

 Research Council Spec. Rept. Ser. 203, 1935, 36.) 



Vegetative growth: Filaments characteristically long, straight, much in- 

 terwoven and ramified; typical unicellular mycelium, usually forming me- 

 dium to large heavy cartilaginous colonies. 



Aerial mycelium: A very slight transient aerial mycelium appeared on 

 one agar slope, but this has not been repeated on any slide microculture on 

 any medium. 



Nutrient agar: Smooth, shining, round, cream-colored colonies, margin 

 submerged, scant white aerial mycelium in 1 week; colonies large (up to 

 10 mm in diameter), centers elevated, greenish tinge, very sparse aerial 

 mycelium in 3 weeks; the aerial mycelium disappears and large radial 

 grooves appear in most colonies in 3 weeks. 



