SPECIES OF STREPTOMYCES 121 



Gelatin: Round cream-colored colonies on surface and a few mm below. 

 No liquefaction. 



Milk: Surface growth green, liquid hydrolyzed, partly clear, purple; later 

 decolorized, brown. 



Glycerol agar: Coiled, colorless, lustrous patches, isolated colony with 

 central depression. 



Potato agar: Colorless blister colonies in 1 week; dull green heaped and 

 coiled mass after 3 weeks; medium becomes slightly discolored. 



Serum agar: Poor growth; small, amorphous, cream-colored mass. 



Source: From pus containing typical actinomycotic granules from parotid 



142. Streptomyces beddardii (Erikson) Waksman and Henrici. (Erik- 

 son, D., Med. Research Council Spec. Rept. Ser. 208, 1935, 36.) 



Vegetative growth: Rapidly growing, dense, spreading mycelium com- 

 posed of very long slender filaments, many wavy or closely coiled, particu- 

 larly on glucose agar; spirals less marked or lacking on poorer nutritive 

 media like synthetic glycerol agar or water agar. 



Aerial mycelium : Sparse, short, straight on synthetic glycerol agar, much 

 slower and more plentiful on glucose agar; later shows long, very fine spirals 

 breaking up into small oval conidia; aerial hyphae straighter and more 

 branched with shorter conidiophores on starch agar. 



Nutrient agar: Colorless, coherent, wrinkled, membranous growth with 

 submerged margin; after 3 months, medium discolored, scant white aerial 

 mycelium. 



Glucose agar: Wrinkled membranous growth; after 2 months, scant white 

 aerial mycelium. 



Potato: Growth moist, membranous, colorless, with scant white aerial 

 mycelium at top of plug. 



Gelatin: Dull white flakes sinking to bottom as medium liquefies; lique- 

 faction complete in 8 days. 



Milk: Coagulation followed by peptonization. 



Starch: Growth spreading, colorless; considerable white aerial mycelium. 

 Hydrolysis. 



Nutrient broth: Suspended and sedimented colorless flocculi, some small 

 round colonies. 



Glycerol agar: Small, cream-colored, discrete colonies becoming con- 

 fluent, under surface much buckled. 



Calcium agar: Growth extensive, moist, wrinkled, membranous, cream- 

 colored. 



Potato agar: Moist, cream-colored skin, convoluted, closely adherent. 



