122 ACTINOMYCETES 



Dorset's egg medium: Growth extensive, very wrinkled, membranous, 

 surface bright yellow. After 2 months, considerable liquefaction. 



Blood agar: Hemolysis. Growth in uniformly striated colorless bands, 

 occasional round colonies at margin. 



Serum agar: Growth wrinkled, glistening, membranous, cream-colored. 



Inspissated serum: Growth smeary, colorless, reverse becoming trans- 

 parent, starting to liquefy at base; completely liquefied and brown in 12 

 days. 



Synthetic glycerol solution: At first, a few round white colonies in sus- 

 pension; later, large, branched, feathery mass at bottom. 



Source: Human spleen in a case of splenic anemia. 



143. Streptomyces kimberi (Erikson) Waksman and Henrici. (Erik- 

 son, D., Med. Research Council Spec. Rept. Ser. 203, 1935, 36.) 



Vegetative growth: Mycelium of long, straight, profusely branching fila- 

 ments forming circumscribed colonies on all media. 



Aerial mycelium: Abundant, short, straight, and branched; small round 

 conidia. 



Nutrient agar: Colonies smooth, round, moist, cream-colored, 1 mm in 

 diameter; after 17 days, aerial mycelium powdery white. 



Glucose agar: Discrete cream-colored colonies becoming confluent; aerial 

 mycelium white. 



Gelatin: Liquefaction. Smooth shining colonies becoming powdery white 

 with aerial mycelium, floating on liquefied medium. No pigmentation. 



Milk: Coagulation; no peptonization; initial pinkish brown ring descends 

 until medium is dark brown throughout ( 2 months). 



Starch: No hydrolysis. 



Nutrient broth: Small, round colonies in sediment in 2 days; superna- 

 tant colonies with white aerial mycelium and large hollow flakes in sediment 

 in 15 days; occasional reddish brown coloration. 



Glycerol agar: Moist cream-colored colonies becoming confluent; aerial 

 mycelium white. 



Calcium agar: Dull, cream-colored, scaly growth, covered by chalky, 

 white aerial mycelium. 



Potato agar: Extensive growth covered by white, powdery aerial myce- 

 lium; large, colorless exuded droplets. 



Wort agar: Heavy, brownish, lichnoid colony; after 30 days, white aerial 

 mycelium. 



Czapek's agar: Small colonies covered with white aerial mycelium. 



Dorset's egg medium: Closely adherent scale-like colonies, centrally ele- 

 vated, with white aerial mycelium. 



