SPECIES OF STREPTOMYCES 123 



Serum agar: Moist, cream-colored honeycombed skin; aerial mycelium 

 scant, white. 



Synthetic glycerol solution: Round white colonies at bottom; later co- 

 herent mulburry-like mass composed of fluffy round portions; after 15 

 days, irregular wispy flocculi and large coherent mass. 



Source: Blood culture of a woman with acholuric jaundice. 



144. Streptomyces somaliensis (Brumpt) Waksman and Henrici. 

 (Brumpt, Arch. Parasitol., Paris, 10, 1906, 489; Precis de Parasitologic, 

 Paris, 2nd ed., 1913, 967.) 



Vegetative growth: Simple branching unicellular mycelium with long 

 straight filaments, forming circumscribed colony. 



Aerial mycelium: Short, straight. 



Nutrient agar: Growth abundant, granular, yellowish, with small dis- 

 crete colonies at margin; later growth colorless, colonies umbilicated. 



Glucose agar: Growth poor, elevated patch, moist, cream-colored. 



Potato: Abundant growth, colonies round and oval, partly piled up in 

 rosettes, frosted with whitish gray aerial mycelium, plug discolored; after 

 16 days, aerial mycelium transient, growth nearly black. 



Gelatin: Cream-colored colonies, medium pitted; complete liquefaction 

 in 10 days; hard black mass at bottom. 



Milk: Soft semiliquid coagulum which undergoes digestion; heavy 

 wrinkled surface pellicle, completely liquefied in 12 days. 



Nutrient broth: A few round white colonies at surface, numerous fluffy 

 masses in sediment; later large irregular mass breaking into wisps. 



Glycerol agar : Growth abundant, minute round to large convoluted and 

 piled up masses, colorless to dark gray and black. 



Calcium agar: Round cream-colored colonies, depressed, umbilicated, 

 piled up; aerial mycelium thin, white; colonies become pale brown. 



Doreset's egg medium: Extensive colorless growth, partly discrete; be- 

 coming opaque, cream-colored, very wrinkled; later rough, yellow, mealy, 

 portion liquid. 



Blood agar: Small dark brown colonies, round and umbilicated, piled 

 up in confluent bands, reverse red-black; hemolysis. 



Serum agar: Spreading yellow-brown skin, intricately convoluted. 



Antagonistic properties: Positive. 



Source: Yellow-grained mycetoma, Khartoum. 



Remarks: Although Streptomyces somaliensis has been known for a long 

 time, there have been, until recently, no detailed descriptions of the or- 

 ganism beyond the fact that it possesses a distinctly hard sheath around 

 the grain which is insoluble in potash and eau de javelle. The rare occur- 



