SPECIES OF NOCARDIA 135 



smooth, shilling, radiate from center, borders cleft. Line of puncture 

 filiform, erose. 



Milk: Pale pink pellicle. 



Nutrient broth: Growth slight, turbid. 



Nitrate: No reduction. 



Dorset's egg medium: After 2 weeks raised, moist, finely granular, 

 irregular margin, flesh-colored. 



Loeffler's medium: After 7 days, growth as on Dorset's egg medium, 

 but pink. 



Potato glycerol: After 7 days, flat, dry, rough, orange-colored. 



Phenol is utilized. 



Temperature: Optimum 25°C. 



Source: Soils in Great Britain. 



Remarks: Differs from Nocardia coeliaca and Nocardia actinomorpha 

 in the filiform growth and absence of liquefaction of gelatin. Long rods 

 and filaments. No acid from glucose, lactose, sucrose, or glycerol. 



6. Nocardia leishmanii Chalmers and Christopherson. (Birt and Leish- 

 man, J. Hyg., 2, 1902, 120; Chalmers, A., and Christopherson, I., Ann. 

 Trop. Med. Parasitol., 10, 1916, 255.) 



Mycelium: Initial cells frequently swollen, large and irregular, aggre- 

 gated in short chains and then branching out into regular narrow filaments ; 

 at margin of colony on synthetic glycerol agar may be seen comparatively 

 long thick segments with accompanying fringe of normal hyphae; later 

 entire colonies asteroid in appearance, very fine and close angular branch- 

 ing, with aerial hyphae situated singly; aerial mycelium generally abundant 

 with irregularly cylindrical conidia. Slightly acid-fast. The latter property 

 must have been attenuated during artificial cultivation, for the organism 

 is reported by the original isolators as markedly acid-fast. 



Glucose agar: Rounded elevated colonies with paler frosting of aerial 

 mycelium; growth becoming piled up, aerial mycelium sparse. 



Gelatin: Small pink colonies in depths of stab. No liquefaction. 



Milk: Surface growth, white aerial mycelium, solid coagulum; later 

 partly peptonized with pink aerial mycelium. 



Glycerol agar: Small round pink colonies, tending to be umbilicated 

 and piled up, stiff white aerial spikes. 



Potato agar: Minute colorless round colonies, small raised patches of 

 white aerial mycelium. 



Dorset's egg medium: Colorless confluent growth studded with little 

 wart-like projections bearing stiff aerial spikes; growth becomes pinkish 

 with white aerial mycelium; later, growth drab-gray, medium discolored. 



