136 ACTINOMYCETES 



Serum agar: Minute, round, colorless colonies with pinkish tinge in 

 confluent raised patch. 



Nutrient broth: Liberal growth, white flocculent colonies; later pink 

 surface colonies. 



Carrot plug: Small, irregularly round, raised colonies, colorless, covered 

 with stiff aerial spikes; later buff-colored, convoluted, and ribbed growth 

 with small patches of white aerial mycelium; aerial mycelium pink in 2 

 months. 



Source: Fatal case of lung disease and pericarditis in man. 



7. Nocardia caprae (Silberschmidt) Waksman and Henri ci. (Silber- 

 schmidt, Ann. Inst. Pasteur, 18, 1899, 841. 



Mycelium: Initial cells only slightly enlarged; early development of 

 aerial hyphae, while substratum threads are still short; frequent slipping 

 of branches; aerial mycelium abundant on all media with tendency to 

 form coherent spikes; mycelium not very polymorphous, but occasional 

 thicker segments appear. Slightly acid-fast. 



Glucose agar: Irregular, bright pink growth tending to be heaped up; 

 later abundant masses frosted over with thin, white aerial mycelium. 



Potato: Abundant growth, small colonies, mostly confluent, entirely 

 covered with pale pink aerial mycelium; growth becomes membranous, 

 considerably buckled ; later superficial colonies with pink aerial mycelium 

 on liquid at base of tube, bottom growth of round white colonies. 



Gelatin: Extensive dull growth with small raised patches of pink aerial 

 mycelium; later ribbon-like, depressed. No liquefaction. 



Milk: Red surface skin; solid coagulum. 



Starch: No hydrolysis. 



Glucose broth: Small sediment of fine flocculi; later, pellicle composed 

 of small pink colonies; superficial skin entire and salmon-colored in 16 days. 



Nutrient broth: Superficial pellicle composed of pink colonies with white 

 aerial mycelium; moderate flocculent sediment. 



Glycerol agar: Abundant growth, small round pink colonies, partly 

 covered with white aerial mycelium. 



Potato agar: Extensive, thin growth, pink in raised patches, covered by 

 white aerial mycelium; later aerial mycelium also becomes pink. 



Starch agar: Minute colorless colonies covered by white aerial mycelium. 



Blood agar: Minute, round, colorless colonies aggregated in broad pink 

 zones, paler aerial mycelium. No hemolysis. 



Dorset's egg medium: Few colorless colonies, some pink, white aerial 

 mycelium; later, growth becoming dull pink, irregular, with scant white 

 aerial mycelium. 



