SPECIES OF NOCARDIA 137 



Calcium agar: Minute colorless colonies, white aerial mycelium; later a 

 pinkish tinge. 



Serum agar: Small, round, pink colonies frosted over with thin, white 

 aerial mycelium. 



Synthetic glycerol solution: Round pink disk-like colonies on surface 

 and tenuous white wispy growth in suspension and sediment; after 20 

 days, surface colonies bearing white aerial mycelium extending 2 cm up 

 tube. 



Source: Lesions in goats. 



8. Nocardia pretoriana Pijper and Pullinger. (Pijper, A., and Pullinger, 

 B. D., J. Trop. Med. Hyg., 30, 1927, 153.) 



Mycelium: Minute, flat colonies consisting of angularly branched fila- 

 ments, and bearing a few short straight aerial hyphae; later, growth be- 

 comes spreading and extensive, the slipping of the branches is well marked 

 and the aerial hyphae are divided into cylindrical conidia. Slightly acid-fast. 



Glucose agar: Pale buff, umbilicated and piled up colonies. 



Potato: Small, raised, pale pink colonies with white aerial mycelium; 

 after 2 months, plug and liquid discolored, growth dull buff, dry and 

 convoluted at base, round and zonate at top of slant, white aerial mvcelium, 

 surface and bottom growth on liquid. 



Gelatin: A few colorless flakes. No liquefaction. 



Milk: Yellowish surface growth; solid coagulum in 1 month; later, partly 

 digested, pale pink growth up the wall of the tube. 



Nutrient broth : Moderate quantity of flakes and dust-like surface growth. 



Glycerol agar: Piled up pink mass, very scant white aerial mycelium 

 at margin. 



Dorset's egg medium: A few round, colorless colonies in 3 days; after 3 

 weeks, irregular, raised pink mass, warted appearance, moderate degree 

 of liquefaction. 



Calcium agar: Yellowish wrinkled coherent growth with white aerial 

 mycelium on apices and at margin. 



Serum agar: Raised, convoluted, slightly pinkish growth. 



Synthetic sucrose solution: A few colorless flakes on the surface, lesser 

 bottom growth. 



Source: A case of mycetoma of the chest wall in a South African native. 



9. Nocardia vacciniae Demarec and Smith. (Demarec, J. B., and Smith, 

 N. R., Phytopathology, 42, 1952, 249-252.) 



Growth: Rods and filaments 0.4-0.8 n in diameter, granular appear- 

 ance when stained and eventually breaking up into bacillary forms, few 

 cells acid-fast. Fat was demonstrated by staining with Sudan black B. 



