138 ACTINOMYCETES 



Synthetic agar: Growth scant, gray. 



Nutrient agar: Growth poor, slow, granular, gray, sometimes pinkish in 

 old cultures. 



Potato: Growth slow, spreading, raised, gray. 



Milk: Growth dry, raised, gray with orange spots; no hydrolysis. 



Potato-yeast-mannitol agar: Growth abundant, fluffy, gray to orange. 



Nitrate: Slow reduction. 



Gelatin-nutrient agar: Dry, ribbon growth; no hydrolysis. 



Starch-nutrient agar : Dry, ribbon, pinkish to orange growth ; hydrolysis 

 positive. 



Citrates: Limited utilization. 



Paraffin: Utilization. 



Temperature: Growth best at 25°-28°C; inhibited at 32°; none or very 

 scant at 37°. 



Antibiotic activity: None. 



Remarks: On a basal agar with ammonia as the source of nitrogen, acid 

 was formed from glucose, sucrose, glycerol, and mannitol; reactions var- 

 iable with arabinose and xylose; no growth on lactose or sorbitol. Re- 

 sembles Nocardia minima (Jensen) Waksman and Henrici, but differs 

 from it in the following respects : Utilizes glycerol and mannitol and some- 

 times arabinose and xylose; reduces nitrates to nitrites; utilizes citrates; 

 and causes formation of bud-proliferating galls on blueberry plants. 



10. Nocardia pulmonalis (Burnett) Waksman and Henrici. (Burnett, 

 S. H., Ann. Rept. N. Y. State Vet. Coll., 1909-1910, 167). 



Mycelium: Gram-positive mycelium breaking up readily into oval-shaped 

 conidia. Acid-fast, especially in early stages of growth. 



Nutrient agar: Moist, raised growth in form of small, spherical colonies. 



Glucose agar: Dull, whitish, convoluted growth. 



Potato: Luxuriant growth in form of small, translucent, round colonies; 

 becoming colored lemon-yellow; later, growth becomes convoluted or folded 

 with chalky white aerial mycelium, color of plug brownish. 



Gelatin: Small, whitish, spherical colonies; edges of colony becoming 

 chalky white; limited liquefaction. 



Milk: Colonies on the surface of the medium; coagulation in a few days; 

 later, digestion. 



Nutrient broth: Delicate, translucent film on surface, becoming corru- 

 rugated with some whitish, spherical colonies in medium. 



Source: Lungs of a cow. 



Remarks: Nonpathogenic for rabbits and guinea pigs. 



