SPECIES OF NOCARDIA 141 



Nitrate: Reduction to nitrite. 



Dorset's egg medium: Filiform, raised, dry, wrinkled, orange. 



Loeffler's medium: Similar to growth on Dorset's egg medium, but pink. 



Potato glycerol: Filiform, raised, dry, wrinkled, yellowish brown to coral- 

 red. 



Temperature: Optimum 22°-25°C. 



Habitat: Soil. 



Remarks: Acid from glycerol and glucose with some strains. No acid 

 or gas from sucrose, maltose, or lactose. Phenol and m-cresol are utilized. 

 Some strains utilize naphthalene (Gray and Thornton). 



14. Nocardia globerula (Gray) Waksman and Henrici. (Gray, P., 

 Proc. Roy. Soc. [Londonl B, 102, 1928, 265.) 



Mycelium: Curved rods and filaments 1 by 2-9 n, with many coccoid 

 cells, especially in old cultures. Rods and filaments frequently irregularly 

 swollen. Not acid-fast. Capsules may be present. Gram-positive. 



Nutrient agar: After 4 days surface colonies irregularly round, 3-5 mm 

 in diameter, convex, white, smooth, shining; edge undulate, erose. After 

 7 days, more convex and of a watery appearance. Deep colonies: after 4 

 days, lens-shaped. Slant: after 3 days, filiform, flat, watery; edge irregular. 



Gelatin: After 19 days, surface colonies irregularly round, 1-2 mm in 

 diameter, convex, light buff, smooth, shining; edge entire. Deep colonies: 

 round, with entire edge. Stab: after 8 days, nailhead, irregularly round, con- 

 vex, pinkish white, smooth, shining; line of stab erose. 



Milk: Alkaline. 



Nutrient and peptone broth: Turbid with viscous suspension. 



Nitrate: No reduction. 



Dorset's egg medium: After 2 weeks, spreading, raised, moist, orange- 

 colored. 



Loeffler's medium: Growth as on Dorset's egg medium, but salmon- 

 colored. 



Potato glycerol: After 24 hours, filiform, moist, smooth, pale pink. 



Indole agar: Blue crystals of indigotin formed. 



Temperature: Optimum 25°-28°C. 



Phenol: Utilized. 



Source: Soil in Great Britain. 



Remarks: This organism resembles most closely Nocardia corallina. It 

 is distinguished by producing a more watery type of surface growth, more 

 nearly entire deep colonies, and more particularly by the production of 

 indigotin from indol. No acid from glucose, lactose, maltose, sucrose, or 

 glycerol. 



