SPECIES OF NOCARDIA 145 



Nitrate: No reduction. 



Dorset's egg medium: Raised, smooth, moist, verrucose, buff-colored. 



Loeffler's medium: After 10 days, slight growth, dry, granular, pale 

 buff -colored. 



Potato glycerol: After 2 days, dry, crumpled, orange-colored, becoming 

 brown after about 10 days. 



Temperature: Optimum 22°-25°C. 



Source: Soil in Great Britain and Australia. 



Remarks: No acid from glucose, lactose, sucrose, or glycerol. Phenol is 

 utilized. Differs from the previously described members of the genus in the 

 absence of chromogenesis. Forms hollow lobes in deep gelatin cultures. 

 Cells are rods, seldom filaments. 



19. Nocardia transvalensis Pijper and Pullinger. (Pijper, A., and 

 Pullinger, B. D., J. Trop. Med. Hyg., SO, 1927, 153.) 



Mycelium: Initial unicellular, but with the central branch frequently 

 broader and showing dense granular refractile contents, small colonies 

 quickly covered with aerial mycelium, the straight aerial hyphae in some 

 cases becoming clustered into irregular spikes, colorless drops are exuded 

 and a pink coloration produced in the densest part of the growth on syn- 

 thetic glycerol agar. Angular branching with division of the substratum 

 filaments can be seen, the aerial hyphae also being irregularly segmented. 

 Acid-fast. 



Nutrient agar: No growth. 



Glucose agar: Raised, granular, pink colonies with white aerial mycelium. 



Potato: Dry, raised, convoluted, pink growth with white aerial mycelium 

 in 1 month; dull, pink, brittle surface colonies, with paler aerial mycelium 

 floating coherently on liquid at base in 2 months. 



Gelatin: Poor growth, a few irregular colorless flakes. No liquefaction. 



Milk: No change. 



Starch: No hydrolysis. 



Nutrient broth: Moderate flaky growth. 



Glycerol agar: Small, pink coiled masses with thin, white aerial mycelium. 



Coon's agar: Colorless growth with liberal white aerial mycelium. 



Dorset's egg medium: Small, irregularly raised, and coiled, dull pink 

 mass. 



Serum agar: Very poor growth. 



Synthetic sucrose solution : Poor growth, a few flakes on surface, a few 

 at bottom. 



Inspissated serum: Scant, colorless, flaky growth; later a minute tuft of 

 pale pink aerial mycelium. 



Source: A case of mycetoma of the foot in South Africa. 



