148 ACTINOMYCETES 



Growth: Colonies colored dark green. Pigment not soluble in medium, 

 in water, or in organic solvents. Surface of colony somewhat shiny. On 

 potato, rough, much-folded, broken up into small colonies. 



Mycelium: Cells often branching, 0.7-0.8 n in diameter with cross-wall; 

 after 5-7 days the cells break up into rods, 5-15 /x long. Cocci not observed. 

 Cells multiply by fission, seldom by budding. Spores not formed. Cells 

 gram-positive, not acid-fast. 



Gelatin: No liquefaction. 



Milk: No peptonization or coagulation. 



Starch: Weak hydrolysis. 



Sucrose: Ready inversion. 



Habitat: Soil. 



Remarks : Good growth on fats and paraffin and less on wax. 



24. Nocardia citrea (Krassilnikov) Waksman and Henrici. (Krassil- 

 nikov, N. A., Bull. Acad. Sci. USSR, No. 1, 1938, 139.) 



Mycelium: In young cultures, very fine threads 0.3-0.5 ju in diameter. 

 After several days the cells break up into short rods 0.5 by 1.5-5 n and into 

 cocci 0.3-0.5 /x in diameter. Multiplies by fission and bud formation; spores 

 not formed. Cells not acid-fast. 



Growth: Yellow-green, usually rough and folded. 



Gelatin: Liquefaction. 



Milk: Coagulation and peptonization. 



Starch: Hydrolysis. 



Sucrose: Inversion. 



Habitat: Soil and water. 



Remarks : Weak growth on fat. No growth on paraffin or wax. 



25. Nocardia madurae (Vincent) Blanchard. (Vincent, H., Ann. Inst. 

 Pasteur, 8, 1894, 129; Blanchard, R., in Bouchard, Traite" Pathol. Gen. 

 2, 1896, 868.) 



Mycelium : Morphology in tissues, growth in form of granules consisting 

 of radiating actinomycosis. In cultures, initial branched mycelium frag- 

 menting into rod-shaped and coccoid bodies. No aerial mycelium or spores. 

 Not acid-fast. 



Potato: Wrinkled friable growth; buff-colored, sometimes red. 



Gelatin: Growth scant, whitish; no liquefaction. Colonies round, glisteri- 

 ng, at first white, then buff to rose or crimson. Pigment production irregular 

 and unpredictable. Occasionally red soluble pigment is produced. Growth 

 eventually wrinkled. No aerial mycelium. 



Milk: No change, or slight, slow peptonization. 



Nutrient broth: Growth as a floccular sediment. 



