SPECIES OF NOCARDIA 149 



Source : Cause of some cases of Madura foot. 



Remarks: Not pathogenic for the usual laboratory animals; pathogenic 

 for monkeys (Musgrave, W. E., and Clegg, M. T., Philippine J. Sci., Ser. 

 B., Med. Sci., S, 1908, 470). 



26. Nocardia nigra (Krassilnikov) comb. nov. (Not N. nigra Castellani 

 and Chalmerson, not Streptothrix nigra Rossi Doria.) (Krassilnikov, N. A., 

 Actinomycetales, Akad. Nauk. USSR, Moskau, 1941, 89.) 



Vegetative growth: Colonies rough, folded, shiny, dough-like consistency. 

 Growth at first colorless or brownish, gradually becoming darker and after 

 10-15 days, dark brown and even black. Pigment not dissolved in medium. 

 It is similar to melanin. Hyphae thread-like breaking up into rods 2-10 by 

 0.7 /x and cocci, 0.6-0.8 /z- No aerial mycelium. 



Synthetic agar: Growth poor. Many cells are swollen to 3 n in diameter. 



Nutrient agar: Growth poor. 



Potato: Good growth. 



Gelatin: No growth or liquefaction. 



Milk: No change. 



Cellulose: No growth. 



Nutrient broth : Small sediment produced. Medium clear. 



Source: Soil. 



Remarks: Seldom found. Culture rapidly disappears on continued cul- 

 tivation. 



27. Nocardia lutea Christopherson and Archibald. (Christopherson, 

 and Archibald, Lancet, 2, 1918, 847.) 



Mycelium: Initial elements swollen and segmented, giving rise to ir- 

 regular, spreading, polymorphous colonies composed of cells of all shapes 

 and sizes with markedly granular contents. Later more monomorphous, 

 the filaments being arranged in angular apposition. Sometimes (e.g., on 

 synthetic glycerol agar) the segments are so granular as to appear banded. 

 On potato agar, small filamentous colonies are formed with irregular angular 

 branching and bear a few isolated short, straight, aerial hyphae. 



Nutrient agar: Abundant, coherent, moist, pink membranous growth 

 with round discrete colonies at margin; after 3 weeks, colorless fringed 

 margin, round confluent portion. 



Glucose agar: Scant, reddish, smeary growth. 



Potato: Carrot-red, moist, thick, granular growth in bands, partly raised 

 and with discrete round colonies ; sparse, colorless, very thin aerial mycelium 

 at top of slant in 2 months. 



Gelatin : Pale pink wrinkled growth on wall of tube and colorless puncti- 

 form and stellate colonies in medium; no liquefaction. 



