150 ACTINOMYCETES 



Milk: Orange-red surface and bottom growth, liquid blue. 



Glucose broth: Abundant, pinkish flaky surface growth, breaking up 

 easily and sinking to bottom. 



Nutrient broth: Pink flakes and surface growth. 



Glycerol agar: Yellowish pink, wrinkled membrane. 



Potato agar: Coherent, pink, moist growth, centrally embedded with 

 small, round, discrete colonies at margin. 



Dorset's egg medium: Poor growth, dull pink, spreading. 



Serum agar : Confluent granular pink membrane. 



Source: Actinomycosis of the lachrymal gland. 



28. Nocardia blackwellii (Erikson) Waksman and Henrici. (Erikson, 

 D., Med. Research Council Spec. Rept. Ser. 203, 1935, 37.) 



Mycelium: Initial elements short, rod-like, growing out into longer forms 

 sparsely branching; small radiating colonies are produced with short, 

 straight aerial mycelium, frequently large, round or ovoid cells are inter- 

 posed in the irregularly segmented chains of cells, being sometimes isolated 

 in company with 2 or 3 short filaments and sometimes terminal. 



Nutrient agar: Confluent wrinkled growth with small, round, pinkish, 

 discrete colonies at margin. 



Glucose agar: Abundant, pale pink growth, small conical colonies, piled 

 up, convoluted. 



Gelatin: Few colorless, minute colonies along line of inoculation; after 

 30 days, abundant colorless colonies to 10 mm below surface, larger pink- 

 yellow surface colonies with white aerial mycelium; no liquefaction. 



Milk: Heavy, convoluted, bright yellow surface pellicle; no coagulation. 



Nutrient broth: Flakes, later innumerable minute colonies, some adher- 

 ing to wall just above liquid level. 



Glycerol agar : Extensive, granular, irregular, thin, pinkish growth ; after 

 40 days, a few discrete colonies with depressed margins, center piled up, 

 pink. 



Potato agar: Small, round, colorless colonies covered with white aerial 

 mycelium; after 2 weeks, colonies dull pink, submerged margins, few aerial 

 spikes, moderate aerial mycelium at top of slant. 



Serum agar: Smooth, cream, umbilicated colonies, with submerged 

 growth extending into medium in scallops 5-8 mm deep; a pale pink mass 

 in 2 weeks. 



Carrot plug: Small, round, smooth, cream-colored elevated colonies in 

 10 days; sparse, stiff, colorless aerial spikes in 16 days; abundantly piled 

 up, convoluted, ocherous growth in 25 days. 



Source: Hock joint of foal. 



