152 ACTINOMYCETES 



Nutrient broth: Abundant, colorless growth; flocculent mass at bottom 

 and pellicle at surface; medium slightly discolored. 



Glycerol agar: Dull, mealy, pink, wrinkled growth; scant, white aerial 

 mycelium at top; medium slightly discolored. 



Potato agar: Small, round, lemon-colored colonies, partly confluent, 

 with white aerial mycelium; later medium discolored light brown; sub- 

 merged growth greenish. 



Dorset's egg medium: Extensive, colorless growth, pale pink aerial my- 

 celium in center; later covered with a powdery, pinkish white aerial my- 

 celium. 



Serum agar: Colonies irregular, small, elevated, cream-colored, fre- 

 quently umbilicated. 



Carrot plug: Small, round, colorless colonies, velvety white aerial my- 

 celium; in 2 months, piled-up, pink, granular mass with warted promi- 

 nences; marginal zone white aerial mycelium and thin all-over central 

 aerial mycelium. 



Source: Human pulmonary case of streptothricosis. 



31. Nocardia caviae Snijders (Snijders, Geneesk. Tijdschr. Ned. Indie, 

 64, 1924, 47, 75). 



Mycelium: Initial segmentation, producing elements of approximately 

 even thickness arranged in angular apposition, and later, long, profusely 

 ramifying threads with strongly refractile protoplasm. Aerial mycelium 

 straight and branching, the aerial hyphae with occasional coiled tips di- 

 vided into cylindrical conidia. 



Glucose agar: Piled-up, convoluted, cream-colored to pale pink growth; 

 white aerial mycelium. 



Potato: Small, colorless colonies; white, powdery aerial mycelium; later, 

 abundant, raised, pale pink, confluent growth, discolored plug; after 2 

 months, raised, buckled, pink colonies with white aerial mycelium floating 

 on liquid at base. 



Gelatin: A few colorless flakes. No liquefaction. 



Milk: Colorless surface growth: white aerial mycelium; coagulation. 



Nutrient broth: Cream-colored, wrinkled surface pellicle extending up 

 wall and breaking easily; moderate bottom growth, flaky; medium dis- 

 colored. 



Glycerol agar: Scanty growth. 



Potato agar: Colorless, spreading growth with dense, white aerial my- 

 celium. 



Dorset's egg medium: Heavily corrugated, pale pink growth with sub- 

 merged margin, dense white aerial mycelium in center; after 3 weeks, color- 

 less transpired drops. 



