SPECIES OF NOCARDIA 153 



Serum agar: Pale pink, wrinkled growth, partly submerged; after 4 

 weeks, piled up with scant, white aerial mycelium, medium discolored red- 

 dish brown. 



Source: Infected guinea pigs, Sumatra. 



32. Nocardia actinomorpha (Gray and Thornton) Waksman and 

 Henrici. (Gray, P., and Thornton, H., Centrbl. Bakt. II, 73, 1928, 88.) 



Mycelium: Long, branching filaments and rods, 0.5-0.8 by up to 10 jw. 

 In older cultures, rods 2-3 n long generally predominate. On some media, 

 extensively branching hyphae occur. Readily stained. Not acid-fast. Gram- 

 positive. 



Nutrient agar: After 11 days, round colonies, 1 mm in diameter, convex, 

 white, granular or resinous; long arborescent processes from the edge. Deep 

 colonies: arborescent burrs; processes about equal to diameter of colony. 

 Slant: filiform, raised to convex, white, rugose, dull; edge undulate, with 

 strong tufted projections below surface. 



Gelatin: After 12 days, round, saucer-like, white colonies, raised rim, 

 edges burred. Liquefaction. Deep colonies: burrs. Stab: after 8 to 14 days, 

 saccate liquefaction, 5-8 mm. 



Nutrient broth: Turbid, or clear with white scum. 



Nitrate: Reduction. 



Dorset's egg medium: After 2 weeks, raised, dry, smooth, salmon-buff 

 growth. 



Loeffler's medium: After 2 days, smooth, moist, warty, salmon-colored 

 growth. 



Potato glycerol: After 2 days, dry, wrinkled, pink to orange growth. 



Temperature: Optimum 25°-30°C. 



Source: A few strains have been isolated from soil in Great Britain and 

 Australia. 



Remarks: Differs from Nocardia coeliaca in saccate liquefaction of gela- 

 tin. Long rods and filaments. No acid from glucose, lactose, sucrose, or 

 glycerol. Phenol and naphthalene are utilized. 



33. Nocardia alba (Krassilnikov) comb. nov. (Krassilnikov, N. A., 

 Actinomycetales, Akad. Nauk. USSR, Moskau, 1941, 73.) 



Vegetative growth: Colonies white, never pigmented, dough-like con- 

 sistency; smooth or folded growth; shiny or pale. True substrate mycelium 

 produced at first, which breaks up into short rods 2.7 by 0.7-0.8 /x, later 

 changing into a mass of coccus-like cells, 0.7-1 y. in diameter. Cells gram- 

 positive, non-acid-fast. Many cells are swollen, others form side buds. No 

 aerial mycelium. 



