SPECIES OF NOCARDIA 155 



Glucose broth: Rather scant growth. Granulated, yellowish sediment; 

 no surface growth. Broth clear. No pigment. No acidity. 



Nitrate: Slight or no reduction with various sources of energy. 



Sucrose: Inversion. 



Sucrose agar: Good growth. Vegetative mycelium superficially spreading, 

 much raised and wrinkled, cracking, white to cream-colored, of a dry, but 

 loose and crumbly, consistency. Aerial mycelium scant, thin, white. Faint 

 yellow soluble pigment after 2-3 weeks. 



Source: Soil. 



35. Nocardia gibsonii (Erikson) comb. nov. (Erikson, D., Med. Re- 

 search Council Spec. Rept. Ser. 203, 1935, 36.) 



Mycelium: Young growing mycelium branches profusely at short inter- 

 vals; later grows out into long, frequently wavy filaments; twisted hyphae 

 also seen on water agar. Power of producing aerial mycelium apparently 

 lost. 



Nutrient agar: Small, cream-colored, depressed, partly confluent colonies, 

 becoming an extensive wrinkled cream-colored skin. 



Glucose agar: Cream-colored, wrinkled, membranous growth. 



Potato: No growth. 



Gelatin: Dull white flakes sinking as medium liquefies; liquefaction 

 complete in 12 days. 



Milk: Coagulation; some peptonization. 



Starch: No hydrolysis. 



Nutrient broth: Sediment of flocculi, some round and fan-shaped colonies. 



Potato agar: Wrinkled, glistening, membranous growth. 



Blood agar, Yellowish confluent bands, irregularly wrinkled, with small 

 discrete colonies, clear hemolytic zone. 



Dorset's egg medium: Small, round, smooth, colorless colonies with 

 conically elevated centers. 



Serum agar: Small, moist, cream-colored colonies growing into medium. 



Source: The spleen in a case of acholuric jaundice. Injected into a 

 monkey, and reisolated. 



36. Nocardia fructifera (Krassilnikov) comb. nov. (Krassilnikov, N. 

 A., Actinomycetales, Akad. Nauk. USSR, Moskau, 1941, 78.) 



Vegetative hyphae: Colonies not compact, mostly of dough-like con- 

 sistency, smooth or rough. Mycelium septated, hyphae breaking up into 

 rods and in some cultures into cocci. Cells gram-positive, non-acid-fast. 



Aerial mycelium: Well developed, whitish to rose-colored. Sporophores 

 long. Straight or weakly wavy, but not spiral-shaped. Oidiospores cylindri- 

 cal, elongated, 1.5 by 0.7m. 



