156 ACTINOMYCETES 



Synthetic agar: Rose-colored to bright red and even red-orange-colored 

 growth. Pigment not dissolved in medium. 



Nutrient agar: Aerial mycelium weakly developed or absent entirely. 



Gelatin: Slow liquefaction. 



Milk: Coagulation and weak peptonization. 



Sucrose: Inversion. 



Starch: Weak hydrolysis. 



Cellulose: Poor growth. 



Fats: Good growth. 



Source: Soil. 



Remarks: One strain was obtained as a mutant of Nocardia rubra. 

 Another strain was changed, after 8 months of cultivation, into a typical 

 Streptomyces. This species is considered as a typical transition point between 

 Streptomyces ruber and Nocardia rubra. 



37. Nocardia africana Pijper and Pullinger. (Pijper, A., and Pul- 

 linger, B. D., J. Trop. Med. Hyg, 30, 1927, 153.) 



Mycelium: Unicellular branching mycelium forming small, dense, pink 

 colonies with short, straight, sparse, white aerial mycelium. 



Nutrient agar: A few flat, pink discoid colonies. 



Gelatin: Irregular pink flakes; no liquefaction. 



Glucose agar: Minute, red, discrete, round colonies and piled-up, paler 

 pink mass with thin, white aerial mycelium. 



Milk : Bright red surface growth, liquid unchanged after 1 month; liquid 

 opaque, reddish purple after 2 months; hydrolyzed, clear wine-red after 3 

 months. 



Nutrient broth: Small pink colonies embedded in coherent, flocculent 



Glycerol agar: After 2 weeks, small, heaped-up, colorless masses with 

 pink tinge around the colorless colonies, margin depressed; after 3 weeks, 

 abundant, piled-up, pale pink growth. 



Potato agar: Bright red growth, small round colonies with colorless 

 submerged margins, and piled-up patches with stiff, sparse, white aerial 

 mycelium. 



Dorset's egg medium: Small, colorless, blister colonies, partly confluent; 

 becoming wrinkled, depressed into medium; slight liquefaction. 



Calcium agar: After 1 week, small, round, colorless colonies with red 

 centers, margins submerged; after 2 weeks, growth bright cherry-red, con- 

 fluent, with colorless margin. 



Serum agar: Irregularly round, raised, wrinkled, colorless colonies; be- 

 coming dry, pink, and flaky; later piled up, brownish, friable. 



