158 ACTINOMYCETES 



39. Nocardia maculata (Millard and Burr) Waksman and Henrici. 

 (Millard, W., and Burr, S., Ann. Appl. Biol., 18, 1926, 580.) 



Mycelium: Filamentous organisms possessing a tough, shiny colony 

 which is cartilaginous, rarely producing an aerial mycelium, though in 

 certain strains, it may occur frequently. Retains the mycelium form for 

 long periods. Not acid-fast. 



Gelatin: Liquefaction. 



Milk: No digestion. • 



Starch: Hydrolysis. 



Paraffin: Not utilized. 



Source: Soil. 



Remarks: In the young colony an orange-yellow to orange-red inter- 

 cellular pigment is produced on all media, which may or may not change to 

 black as the culture ages. 



40. Nocardia rhodnii (Erikson) Waksman and Henrici. (Erikson, D., 

 Med. Research Council Spec. Rept. Ser. 208, 1935, 37.) 



Mycelium: In early stages, the minute colonies are composed of hyphal 

 segments arranged in angular apposition; the aerial mycelium being short 

 and straight. Later the growth becomes extensive and spreading, made up 

 partly of long, genuinely branching filaments and partly of short segments 

 exhibiting slipping branching, each giving rise to aerial hyphae. After 2 

 weeks the angular branching is very marked, delicate, spreading, herring- 

 bone patterns being formed. 



Glucose agar: Abundant, coral-pink, convoluted, piled-up growth. 



Gelatin: Rapid liquefaction; pale pink colonies in superficial pellicle 

 and sediment. 



Milk: Bright orange growth; medium unchanged. 



Glucose broth: Thin, pink superficial pellicle, easily breaking up, and 

 small flakes in sediment; after 2 weeks, abundant growth extending up tube. 



Nutrient broth: Salmon-pink flakes in sediment and colonies on surface; 

 after 2 weeks, abundant growth, discoloration of medium. 



Czapek's agar: Minute, colorless, round colonies. 



Glycerol agar: Extensive growth, dull pink colonies, round and umbili- 

 cated, becoming piled up and deeper coral; later partly submerged. 



Potato agar: Abundant, pink growth, piled up, scant, stiff, white aerial 

 mycelium at top of slant. 



Dorset's egg medium: Salmon-pink, granular membrane; later piled up. 



Serum agar: Extensive, reddish, confluent mass, granular, tending to be 

 piled up; the medium around the growth shows reddish coloration in 2 

 weeks. 



Source: From reduvid bug, Rhodnius prolixus. 



