160 ACTINOMYCETES 



Glycerol agar: Extensive, golden brown, convoluted, thin layer. 



Blood agar: Innumerable small, yellowish, ring-shaped colonies; no 

 hemolysis. 



Dorset's egg medium: Minute, cream-colored, elevated colonies, becom- 

 ing golden brown, raised, convoluted. 



Calcium agar: Yellow, scale-like, closely adherent colonies; scattered 

 white aerial mycelium. 



Serum agar: Golden brown, ring-shaped and coiled, smooth colonies; 

 no liquefaction. 



Synthetic glycerol solution: Light, white, fluffy colonies, minute and in 

 clusters. 



Source: Human spleen in a case of acholuric jaundice. 



43. Nocardia kuroishi Uesaka (Uesaka, I., J. Antibiotics [Japan], 5, 

 1952, 75-79). 



Mycelium gives a weak-acid-fast reaction, but the separated cells, 0.8 

 by 1.3 /*, are non-acid-faet. 



Aerial mycelium: Abundant, branching hyphae slightly curved at first, 

 later turning around each other; cells refractive. Granules soon become 

 visible. 



Synthetic glycerol agar: Thin, pale yellow growth, covered partly with 

 punctiform, white aerial mycelium. Yellow pigment. 



Nutrient agar: Wrinkled, grayish yellow colonies. No aerial mycelium. 

 Faint grayish brown soluble pigment. 



Glucose agar: Abundant growth, at first yellowish brown then reddish 

 brown. Scant white aerial mycelium at margin of colonies. Red to wine- 

 colored soluble pigment. 



Potato: Moderate growth, at first red or brownish red, later dark brown. 

 Aerial mycelium grayish white. Dark brown pigment. 



Gelatin: Yellowish brown growth sinking into medium. No aerial my- 

 celium. No liquefaction. Yellowish brown soluble pigment. 



Milk: No coagulation. Slow liquefaction. Brown pigment. 



Starch: Hydrolysis. 



Glucose broth: Red colonies forming pellicle. Abundant, flocculent sedi- 

 ment. Dark brown, soluble pigment. 



Nitrate: No reduction. 



Synthetic solution: White, minute colonies on surface. Medium becomes 

 brown. 



Antagonistic properties: Produces an antibiotic. 



Source: Soil. 



Remarks: Lactose forms excellent source of carbon. 



