ANTIBIOTICS OF ACTINOMYCETES 227 



methanol and slightly soluble in ethanol, butanol and acetone. Biuret, 



ninhydrin, Molisch and Benedict's tests negative. 

 Biological activity: Slight activity against, Micrococcus pyogenes var. aureus, 



no activity against B. subtilis, E. coli and C. albicans. In vivo action 



against Yoshida sarcoma in rats and Ehrlich carcinoma in mice. 

 Toxicity: LD 6 o (mice), intravenous, more than 2,400 mg/kg. 

 Utilization: Not known. 

 Reference: Umezawa, H., Takeuchi, T., and Nitta, K., J. Antibiotics 



(Japan), A 6, 1953, 101. 



Streptin 



Produced by: Streptomyces sp. resembling S. lavendulae and S. reticulus-ruber. 



Method of extraction: Same as streptothricin. 



Chemical and physical properties: Basic, water-soluble substance. 



Biological activity: Active against gram-positive bacteria, gram-negative 

 bacteria, and mycobacteria. More active against staphylococci and 

 micrococci than are streptomycin and streptothricin. 



Toxicity: No data. 



Utilization: None. 



Reference: Woodruff, H. B., and Foster, J. W. ; J. Bacterid., 52, 1946, 502. 



Streptocin 



Produced by: Streptomyces griseus. 



Method of extraction: Extraction of the mycelium with diethyl ether. 

 Concentration of ether in vacuo, extraction of residue with ethanol. 

 Concentration of ethanol, extraction with petroleum ether. Chroma- 

 tography on alumina and elution with 1 per cent acetic acid in methanol 

 yield needle-like crystals. 



Chemical and physical properties: Soluble in water, methanol, and ethanol 

 but not in chloroform. Stable. Sublimable. 



Biological activity: Slightly active against gram-positive bacteria and myco- 

 bacteria. Active against trichomonads. 



Toxicity: LD (mice), intravenous, >800 mg/kg. 



Reference: Waksman, S. A., Harris, D. A., Kupferberg, A. B., Singher, H. 0., 

 and Styles, H., Proc. Soc. Exptl. Biol. Med., 70, 1949, 308-312; 

 Kupferberg, A. B., Styles, H., Singher, H. O., and Waksman, S. A., 

 J. Bacteriol., 59, 1950, 523-526. 



Streptolin 



Produced by: Streptomyces sp. 



Method of extraction: Adsorption on and elution from Darco G60. 



