Contents 



Preface 



Vll 



Chapter I. Introductory. Precautions. Practical Hints 



Chapter II. Staining Methods. General Principles. General Bacterial 

 Stains. Negative Staining of Bacteria. The Gram Stain. Acid-fast 

 Staining. Spore Staining. Staining the Diphtheria Organism. Fla- 

 gella Staining. Capsule Stains. Stain for Fat Droplets. Robinow's 

 Stains for Nuclear Apparatus. Stains for Spirochaetes. Dye Solubil- 

 ities, by H. J. Conn, J. W. Bartholomew, and M. W. Jennison 



10 



Chapter III. Preparation of Media. Introductory. Cultivation and 

 Storage Media. Enrichment Media. Differential Media. Media 

 for Determination of Physiological Properties. Media for Special 

 Bacteriological Procedures. Media for Special Purposes. Appendix: 

 Specifications for Bacteriological Grade Agar, Gelatin, and Peptone. 

 byG. W. Burnett, M. J. Pelczar, Jr. and H.J. Conn 37 



Chapter IV. The Measurement of pH, Titratable Acidity, and Oxidation- 

 Reduction Potentials. The Measurement of pH. Titratable Acidity. 

 Buffer Action, and pH Adjustment of Culture Media. The Measure- 

 ment of Oxidation-Reduction Potentials, by Barnett Cohen ... 72 



Chapter V. Maintenance and Preservation of Cultures. Introduc- 

 tion. Selection of Materials. Dormant Conservation. Maintenance 

 Methods. Media Commercially Available. Media Recommended. 

 by Freeman A. Weiss 99 



Chapter VI. The Study of Obligately Anaerobic Bacteria. Anaerobic 

 Culture Methods and Equipment. Technics for the Study of Anaer- 

 obic Bacteria, by L. S. McClung and Robert B. Lindberg .... 120 



Chapter VII. Routine Tests for the Identification of Bacteria. Intro- 

 duction. The Descriptive Charts. Determining Optimum Condi- 

 tions for Growth. Incubation. Variation. Micromethods. Cul- 



