X CONTENTS 



tural Characteristics. Study of Cell Morphology. Relation to Free 

 Oxygen. Action on Nitrates. Chromogenesis. Indole Production. 

 The Production of Hydrogen Sulfide. Liquefaction of Gelatin. 

 Cleavage of Sugars, Alcohols, and Glucosides. Hydrolysis of Starch. 

 The Methyl Red and Voges-Proskauer Tests. Acid Production in 

 Milk. Rennet Production, by H. J. Conn, M. W. Jennison, and 0. 

 B. Weeks 140 



Chapter VIII. Physiological and Biochemical Technics. Introduction. 

 Growth Measurements. Preparations of Cells and Extracts. Bio- 

 chemical Technics, by R. D. DeMoss and R. C. Bard 169 



Chapter IX. Serological Methods. Introduction. Preparation of Anti- 

 serum. Agglutination. Precipitation. Complement-fixation Test 

 for Microbial Antigens, by E. Edward Evans 199 



Chapter X. The Detection of Bacterial Pathogenicity. Introduction. 

 General Factors Underlying Virulence. Preparation of Inocula. 

 Selection and Preparation of Laboratory Animals for Pathogenicity 

 Studies. Methods of Inoculation. Care of Experimental Animals. 

 Clinical Signs in Animals. Recovery of Bacteria from Infected Ani- 

 mals. Detection of Antigen in Tissues. Conclusion. Chart of 

 Identification Procedures, by Joel Warren 224 



Chapter XI. Virological Methods. Introduction. Precautions. Stains. 

 Cultivation: Tissue Cultures. Hemagglutination. Serological Proce- 

 dures. Complement Fixation. Concentrating Viruses. Storage. 

 by Albert P. McKee 246 



Chapter XII. Inoculations with Bacteria Causing Plant Disease. Intro- 

 duction. Simple Representative Inoculation Methods. Treatment 

 with Bacterial Products. Antibody Production. Cognate Considera- 

 tions. Records. Interpretation of Results, by A. J. Riker . . 286 



Chapter XIII. Glossary of Terms Used on the Charts and in the Manual 299 



Index 307 



