10(3 MANUAL OF MICROBIOLOGICAL METHODS 



Table 13. Maintenance Methods (American Type Culture Collection) 



Genus 



Acetobacter 



Acetobacter 



Achromobacter . . . . 

 Achromobacter . . . . 

 Actinomyces 



Aerobacter 



Agrobacterium . . . . 



Alcaligenes 



Arthrobacter 



Azotobacter 



Azotobacter 



Bacillus . . . 



Bacillus 



Bacillus 



Bacter aides 



Brucella 



Cellulomonas 



Cellvibrio 



Chro7nobacterium. . 

 Clostridium 



Corynebacterium . . 

 Corynebacterium . . 

 Corynebacterium . . 

 Corynebacterium . . 



Desulfovibrio 



Diplococcus 



Erwinia 



Erwinia 



Erysipelothrix . . . . 



Escherichia 



Flavobacterium . . . . 



Gaffkya 



Hemophilus 



Hemophilus 



Klebsiella 



Lactobacillus 



Leuconostoc 



Listeria 



Malleomyces 



Micrococcus 



(including 



Staphylococcus) 

 Mycobacterium. . . 

 Mycobacterium. . , 



Moraxella 



Neisseria 



Neisseria 



Neisseria 



Nitrobacter 



Nitrosomonas. . . . 



Species 



Glucose positive 



Mannitol positive 



Various 



Fischeri 



Bovis 



Various 



Various 



Various 



Various 



Glucose positive 



Mannitol positive 



Various 



Starch-hydrolyz- 



ing 

 Urea-decomposing 



Various 

 Various 

 Various 

 Vulgaris 

 Various 

 Various 



Acnes 



Diphtheriae 

 Animal sources 

 Plant pathogens 



Pneumoniae 



Various 



Tracheiphila 



Insidiosa 



Various 



Various § 



Tetragena 



Influenzae 



Pertussis 



Various 



Various 



Various 



Various 



Mallei 



Various 



Saprophytic 



Tuberculosis 



Various 



Gonorrhoeae 



Meningitidis 



Saprophytic 



Agile 



Europeae 



Medium 

 formula No. 



2,3 



4 



23t 



23a 



10 (stab cul- 

 ture under 

 seal) 



23t 



23 1 



23 1 



23t 



5 



6 



23 1 



27 



36 



8,10 



26 



23t 



12 



23 1 



7.17 



10 



10,20 



8,10 



23,25 



30 



8 



23 1 



20 



8 



23t 



1 



20 



8,24 



9 with blood 



20 



17,33,34 



17,33 



10 



16 



23t 



16 



14,18 



8 



1 1 with CO2 



8,20 



10,13,20 



21 



22 



Transfer 

 time 



1 mo 

 1 mo 



1 to 2 mo 

 1 mo 

 1 mo 



2 mo 



2 mo 



3 mo 



3 mo 



4 mo 

 4 mo 



12 mo-indefi- 

 nite 



12 mo-indefi- 

 nite 



12 mo-indefi- 

 nite 



1 mo 



6 wk 



3 mo 



1 mo 



6 wk 



6 mo-indefi- 

 nite 



1 mo 



1 mo 



1 mo 



1-2 mo 



6 mo 



3 mo 



1 mo 



1 mo 



3 mo 



3 mo 



3 mo 



1 mo 



2 mo 

 2-4 wk 

 6 wk 



4 mo 

 1 mo 



3 mo 



4 mo 

 3 mo 



10 days 

 10 days 

 1 mo 

 1 mo 

 1 mo 



Incubation 

 temp, °C 



28 



28 



Room 



Room 



34-37 



30 



Room 



34-37 



Room 

 Room 

 Room 

 28J 



28 



28 



37 



34-37 



30 



Room 



Room 



28 



34-37 



35-37 



30 



Room— 28 



34-37 



35-37 



Room 



Room 



34-37 



34-37 



30 



35-37 



35-37 



35-37 



34-37 



34-37 t 



Room 



34-37 



34-37 



30t 



30 1 



34-37 



34-37 



35-37 



35-37 



34-37 



Room 



Room 



Storage 

 temp, °C 



10 



10 



10 



Room 



Room 



10 

 10 

 10 

 10 

 10 

 10 

 10 



10 



10 



28 

 10 

 10 

 10 

 18 

 Room 



18 

 18 

 18 

 18 

 10 



10 



Room 



10 



10 



10 



10 



10 

 10 

 10 

 10 

 10 

 Room 



10 

 10 

 10 



35-37 

 35-37 

 Room 

 Room 

 Room 



