110 MANUAL OF MICROBIOLOGICAL METHODS 



10. Brain- Heart Infusion Agar 



Brain-heart infusion agar (Difco) 37 g 



Agar 15 g 



(for semisolid) 2 g 



Distilled water 1,000 ml 



pH7.4 



11. Chocolate Agar 



Heat blood agar (formula 8) to 80°C; reslant. 



12. Cellvibrio Medium 



NaNOs 2.0 g 



MgS04 0.5 g 



KCl 0.5 g 



Fe2(S04)3 0.01 g 



KH2PO4 0.14 g 



K2HPO4 1.2 g 



Yeast extract 0.02 g 



Agar 7.5 g 



Distilled water 1,000 ml 



Place a strip of filter paper 3^^- by 4-5 cm on the agar slope, or suspend in liquid 

 medium. 



13. Cystine-trypticase Medium 



Cystine-trypticase agar (BBL) 29.5 g 



Carbohydrate (optional) 5-10 g 



Distilled water 1,000 ml 



Mix well; heat gently with agitation; boil for 1 min. Tube, and autoclave 15 

 min at 12 lb. Store at room temperature. 



14. Dorsett Egg Medium 



Commercial preparations are advised. 



15. Emerson Medium 



NaCI 2.5 g 



Peptone 4.0 g 



Yeast extract 1.0 g 



Beef extract 4.0 g 



Distilled water 1,000 ml 



Adjust to pH 7.0 with KOH; add 



Glucose 10 g 



Agar 30 g 



16. Glycerol Agar 



Blood base agar (Difco) 20 g 



Nutrient agar 15.5 g 



Distilled water 1,000 ml 



Heat to 100°C; adjust pH to 7.3; add 



Glycerol 60 ml 



