114 MANUAL OF MICROBIOLOGICAL METHODS 



30. Sporovibrio Medium (Starkey) 



Peptone 5.0 g 



Beef extract 3.0 g 



Yeast extract 0.2 g 



MgS04 1.5 g 



NazSO* 1.5 g 



Ferrous ammonium sulfate 0.1 g 



(increase to 0.2 g if medium does not blacken after growth) 



Agar 15 g 



Tap water 1,000 ml 



Heat to 100°C, adjust pH to 7.4, add 



Glucose 5 g 



Tube for stabs, using long (150-mm) tubes. Boil tubes to be inoculated for 10 

 min; cool quickly. After inoculating, push cotton plug down about half way, place 

 two pieces (0.5 mm) of pyrogallic acid on it, add 8 to 10 drops of strong NaOH. 

 Cork tightly with rubber stopper, invert, and incubate at 37°C. 



31. Thiobacillus Thioparus Medium 



(a)K2HP04 2.0 g 



CaCh 0.1 g 



MgS04 0.1 g 



MnS04 Trace 



FeS04 Trace 



Tap water 900 ml 



pH7.8 



Dispense 90 ml in 250 ml-flasks; autoclave. 



(6)Na2S203 10 g 



Tap water 50 ml 



Autoclave in flasks. 



(c)(NH4)2S04 0.1 g 



Tap water 50 ml 



Autoclave in flasks. 



At time of inoculating add 5 ml aseptically of each of (6) and (c) to (a) 



32. Thiobacillus Thiooxidans Medium 



(NH4)2S04 0.2 g 



MgS04 0.5 g 



CaCh 0.25 g 



FeS04 Trace 



KH2PO4 3.0 g 



Precipitated sulfur 10 g 



Tap water 1,000 ml 



One-gram portions of sulfur are placed in 10 dry 250-ml flasks, and 100 ml of a solu- 

 tion of the other components is carefully poured into each flask so that the sulfur 

 floats. Sterilize in flowing steam for % hr on 3 consecutive days. 



