MAINTENANCE AND PRESERVATION OF CULTURES 



117 



Table 14. Commercially Available Media Recommended for 

 Culture Maintenance {Continued) 



I Recommendations of 



Organism 



Micrococci. 



Mycobacteria . 



Nonpathogens. 



Neisseria. 



Gonococcua. 



Pasteurella- 

 Listena 

 P. multocida. 

 P. tiUarensis . 



Pleuropneumonia 



Pneumococci. 



Baltimore Biological Laboratory, Inc. 



Media 



CTA medium 



Trypticase soy 



agar with oil 



Dorset medium 

 ATS medium 

 Lowenstein- 

 Jensen medium 



Same, or CTA 

 medium or 

 trypticase soy 

 agar with oil 



CTA medium 



CTA medium 



CTA medium 

 Cystine heart 

 agar with blood 



CTA medium 

 with inacti- 

 vated sheep 

 serum 



PPLO agar with 

 serum 

 CTA medium 



Storage 

 temp, C 



Room 



Room or 

 refriger- 

 ator 



Room 



35 



Room 

 Room 



35 



Room 



Suggested 

 transfer 

 interval 



6-12 mo 



3 mo 



10 days 



3-6 wk 

 1 mo. 



10-21 days 



Difco Laboratories, Inc.* 



Media 



Cooked-meat 

 medium 



Brain-heart infu- 

 sion, or same 

 with 0.2 or 1.5 

 per cent bacto 

 agar 



Blood agar base 



Dorset medium 



Petragnani 

 medium 



Lowenstein 

 medium 



Glycerol agar 



Dextrose starch 

 agar 37 3-2 

 strength + sup- 

 plement C 



GC medium base 

 with hemoglobin 

 -|- supplement 

 C 



Cooked-meat 

 medium 



Tryptose agar 



Cystine heart 



agar -f bacto- 

 hemoglobin 



PPLO agar with 

 ascitic fluid or 

 PPLO serum 

 fraction 



Cooked-meat 

 medium 



Brain-heart infu- 

 sion agar 



Tryptose blood 

 agar base -|- 

 blood 



Stock culture 

 agar 



Storage 

 temp, °C 



Room 



Room 



Suggested 

 transfer 

 interval 



3 mo 



Room 



37 



Room 



1 year 



plus 



4 wk 



1 year 



1 wk 



* Trade names of all products of this company include the prefix "Bacto-." It is omitted in this 

 table to economize on space. 



t Media and storage times and temperatures vary with organisms. Periods of transfer may vary with 

 type of closure of container and concentration of media. Dehydration occurring in cotton-plugged 

 lubes may impair longevity of cultures. 



