Index 



Acetobacier agar, with liver extract, 108 

 Acid and alkali, determination of, 173 

 Acid-base indicators, 40, 78-79 



ionization curves of, 79 

 Acid dyes, 10 

 Acid-fast staining methods, 151 



Cooper's, 20 



fluorescent, 19 



Gross' cold, 18 



Much's, 19 



Ziehl-Neelsen, 18 

 Acid formation, 159-162, 188-190 



in milk, 165 

 Acidity, titratable, 87-90 

 Action on nitrates, 153-154 

 Adjuvants, 201 

 Aerobes, obligate, 38 



use of, to absorb oxygen, 124 

 Agar, Acetobacter, 108 



Ayers & Johnson, 108 



Azotobacter, 108 



beef-extract, 42 



beef-extract peptone, 159 



blood, 50 



chocolate, 50 



double sugar, 54 



egg-yolk, 50 



Endo's, 51 



eosin-methylene-blue, 51 



glycerol, 110 



nutrient, 42, 46, 159 



peptone, 46 



semisolid, 45, 53 



sodium caseinate, 43 



specifications for, 39, 64-67 



starch, 55 



tomato-juice, 115 



triple sugar, 54 



Agar, trypticase soy, 51 



tryptone-glucose-yeast, 115 



tryptose, 51 



urea, 115 



V-8 juice, 115 



yeast-extract-mannitol, 46 



yeast-infusion-glucose, 46 

 Agglutination, 206-209 



quantitative measurement of, 208 



slide tests, 207-208 



tube test, 207 

 Agglutinin absorption, 205 

 Albert's diphtheria stain, 22 

 Albumin, egg, coagulated, 55 

 Alcohols, cleavage of, 159-162 

 Alkaline egg medium, 55 

 Alkaline pyrogallol for oxygen removal, 



126 

 Allantoic fluid, harvesting, 263 

 Allantoic sac in virus cultivation, 260 

 American Type Culture Collection, 99 

 Amino acid metabolism, 191-193 

 Ammonium oxalate crystal violet, 13, 16 

 Amniotic fluid, harvesting, 263 

 Amniotic sac in virus cultivation, 259 

 Anaerobes, beef liver for, 109 



cultivation of, 132-135 



demonstration of spores, 131 



enrichment of, 133 



examination of, microscopic, 129-137 

 for motility, 130 



facultative, 38 



flagella, staining of, 28 



granulose reaction of, 132 



inoculation, technics for, 135 



isolation of, 134 



media for, 49 



obligate, 38, 120-139 



307 



