308 



MANUAL OF MICROBIOLOGICAL METHODS 



Anaerobes, purification of, 133 



serological reactions of, 137 



study of, 120-139 

 technics for, 129-137 

 Anaerobic dish. Bray's, 126 



Spray's, 126 

 Anaerobic jar, Brewer's, 122 

 Anaerobiosis, methylene blue as indicator 



of, 122 

 Anilin "gentian violet," 14 

 Animals, autopsy of, 256 



clinical signs in, 234 



experimental, care of, 234 



laboratory, 229-230 



recovery of bacteria from, 235, 244 

 Anthony's method of capsule staining, 29 

 Antibiotics, in plants, 296 



in virus cultivation, 252 

 Antibodies, 199-201 



for complement fixation, 276 

 Antibody absorption, 204 

 Antibody production in plants, 294 

 Antibody purification, 203 

 Antigen test, dilution method for precipi- 

 tation, 210 

 Antigen titration, 220 

 Antigens, 199-201 



for agglutination, preparation of, 209 



anticomplementary activity of, 219 



for complement-fixation test, 218, 275 



hemolytic activity of, 219 



protein, 204 

 soluble, 201 



in tissues, detection of, 214 

 Antihemagglutination by viruses, 



271-274 

 Antiserum, 199, 219 



harvesting of, 202 

 Assay, microbiological, 192, 195 

 Autopsy of animals, 236-244, 256 

 Autotrophic organisms, 38 

 Ayers & Johnson agar, 108 

 Azotobacter agar, 108 



Bacterial genetics, 195 



Bacterial products, treatment of plants 



with, 293 

 Bacterial stains, general, 13-14 

 Bacteriophages, 246 

 Bacteriostasis, use in enrichment media, 



50 



Bartholomew and Mittwer's "cold" 



method for spore staining, 21 

 Basic dyes, 10 

 Beef-extract agar, 42 

 Beef -extract peptone agar, 159 

 Beef -extract peptone broth, 42, 159 

 Beef-heart infusion, 55 

 Beef liver for anaerobes, 109 

 Beef -liver infusion, 44 

 Benians' congo red, 15 

 Biochemical mutants, 195 

 Biochemical and physiological technics, 



169-198 

 Biochemical technics, 181-195 

 Biological methods for oxygen removal, 



123-125 

 Blood, peptic digest of, 112 

 Blood agar, 50, 109 

 Blood glucose agar, 109 

 Blood serum, coagulated, 52 

 Bordet-Gengou medium, 109 

 Brain-heart infusion agar, 110 

 Brain medium, 44 

 Bray anaerobic dish, 126 

 Brewer anaerobic jar, 122 

 Brewer culture dish, 128 

 Brom cresol green, 40, 78-79, 160-161 

 Brom cresol purple, 40, 54, 78-79, 



160-161 

 Brom phenol blue, 40, 78-79, 161 

 Brom phenol red, 40 

 Brom thymol blue, 40, 54, 78-79, 90 

 Broth, beef-extract peptone, 42, 159 



infusion, 43 



liver-infusion, 46 



nitrate, 56 



nutrient, 42 



thioglycollate, 43 

 Brucella, media for, 51 

 Buffer action, 89 



Buffer mixtures, composition of, 82-83 

 Buffer solutions, 76, 81-83 

 Burdon's stain for fat droplets, 30 

 Burke and Kopeloff-Beerman modifi- 

 cations of gram stain, 16 

 Burke's iodine solution, 16 



Calomel electrode for pH measurement, 



73 

 Calomel half-cell, 92 

 standardization of, 74 



