310 



MANUAL OF MICROBIOLOGICAL METHODS 



Diphtheria stain, 22-24 



Albert's, 22 



Kinyoun's, 24 



Ljubinsky's, 23 



Neisser's, 23 



Ponder's, 24 

 Direct microscopic method, 171 

 Dissimilation, products of, 159-165, 



183-187 

 Dissociation, 6 

 Dormant conservation of cultures, 



101-115 

 Dorner's method for spore staining, 20- 



21 

 Dorner's nigrosin solution, 15 

 Dorsett egg medium, 110 

 Double sugar agar, 54 

 Drop-ratio color standards, Gillespie's, 



84-86 

 Dry weight of cells, 172 

 Drying of cells, methods for, 176 

 Dye solubilities, 32-34 

 Dyes, acid and basic, 10 



Experimental animals, care of, 234 

 Extracts, preparation of, 174-181 



Facultative anaerobes, 38 



Fat droplets, stain for, 30 



Fermentation tests, medium for, 53-55 



Fermentation tube, 161 



50 per cent infectious dose for embryos, 



266 

 Filtration of viruses, 251 

 Flagella stains, 24-28, 131, 150 



for anaerobes, 28, 131 



Bailey's, 27 



Casares-Gil's, 26 



Gray's, 26 



Leifson's, 27 

 Fluorescence method for acid-fast stain- 

 ing, 19 

 Fontana stain for Spirochaetes, 31 

 Freezing, deep, 105 

 Fuchsin, carbol, Kinyoun's, 14 



Ziehl's, 13 

 Fungus inoculation of plants, 292 



Egg albumin, coagulated, 55 

 Egg medium, alkaline, 55 

 Egg-yolk agar, 50 

 Eggs, candling of, 258 



embryonated, harvesting fluids and 

 membranes, 262-264 



post-inoculation incubation of, 261 

 Eh measurements, significance of, 93 

 Electrodes and electrode vessels for 



oxidation-reduction determinations, 

 91 

 Electron microscope, 248 

 Embryonated eggs, harvesting fluids and 



membranes, 262-264 

 Embryos, chicken, cultivation of viruses 



in, 257-267 

 Emerson medium, 110 

 Endo's agar, 51 

 Endospores, 151 

 Enrichment media, 46 

 Enzyme, receptor-destroying, 274 

 Enzyme extracts, cell-free, 177 

 Eosin-methylene-blue agar, 51 

 Erythrocytes, preparation of, 215 



sensitization of, 216 



sheep, preparation of, 215 

 Equivalence point of indicator, 87 



Gamma globulins, 204 



Gas production, measurement of, 161 



Gelatin, hydrolysis of, 55 



liquefaction of, 158 



media to demonstrate, 55 



nutrient, 55 



specifications for, 39, 67-69 



standards for, 39 

 Gels, precipitation in, 213 

 Genetics, bacterial, 193 

 Gentian violet, anilin, 14 

 Giemsa's stain, 249 



Gillespie's drop-ratio standards, 84-86 

 Glass electrode, 75-77 



standardization of, 76 

 Globulins, gamma, 204 

 Glossary of terms, 299-305 

 Glucosides, cleavage of, 159-162 

 Glycerol agar, 110 



Gram-negative nonsporeforming bac- 

 teria, media for, 51 

 Gram stain, 15-18, 151 



interpretation of, 17 



modification of, Burke's, 16 

 Hucker, 16 

 Kopeloff-Beerman, 16 



