312 



MANUAL OF MICROBIOLOGICAL METHODS 



Lactic acid bacteria, media for, 48 

 Leifson's capsule stain, 28 

 Leifson's flagella stain, 27 

 Lipolysis, 57 

 Liquefaction of gelatin, 158 



media to demonstrate, 55 

 Litmus milk, 56 

 Liver-infusion broth, 46 

 Liver medium, 111 

 Ljubinsky stain for diphtheria, 23 

 Loeffler's alkaline methylene blue, 13 

 Lowenstein-Jensen medhun. 111 

 Lugol's solution. Gram's modification of, 



16 

 Lyophilization, 101-104 



milk solution for, 100 



Macchiavello's stain for Rickettsiae, 



31, 249 

 Macroscopic slide test for agglutination, 



208 

 Maintenance and preservation of 



cultures, 99-119 

 Maintenance media, 108-118 

 Maintenance methods (ATTC), 106-107 

 Manometers, 182 

 Manometric technics, 181-183 

 Manual, scope of, 1 

 "Manual of Methods for Pure Culture 



Study of Bacteria," 2 

 Measurement, of oxidation-reduction 

 potentials, 91-98 

 colorimetric method, 94-98 

 potentiometric method, 91-94 

 of pH, 72-187 



colorimetric method, 77-87 

 potentiometric methods, 72-77 

 Meat-infusion broth, 111 

 Media, alkaline egg, 55 

 for anaerobic bacteria, 49 

 for Brucella, 51 

 carbohydrate, 54 

 carbohydrate indicator, 53 

 for coliform group, 48 

 corn liver, 49 



for Corynehacierium diphtheriae, 52 

 cultivation, 41-46 

 dehydrated, 41 



to demonstrate gelatin liquefaction, 55 

 for determination, of physiological 

 properties, 53-57 



Media, for determination, of utilization 

 of citrate, 56 



differential, 50-53 



enrichment, 46 



for fermentation tests, 53 



for gram-negative nonsporeforming 

 bacteria, 51 



for H2S production, 56 



indicator, 160 



for indole determination, 56 



for isolation of Staphylococci, 51 



for lactic acid bacteria, 48 



maintenance, 108-115 



for metabolically active cells, 58 



for Mycobacterium tuberculosis, 52 



for Neisseria gonorrhoeae, 52 



for nonsulfur photosynthetic bacteria, 

 47 



for pigment production, 57 



preparation of, 37-70 



reaction of, 39 



recommended for culture maintenance, 

 116-118 



for special purposes, 57-58 



for specific bacteriological procedures, 

 57 



storage, 45 



sugar, 160 



for sulfur-oxidizing bacteria, 47 



synthetic, 38, 54 



for Voges-Proskauer reaction, 56 

 Metabolically active cells, harvest of, 174 



media for, 58 

 Methyl orange, 78-79 

 Methyl red, 40, 78-79 



and Voges-Proskauer tests, 163-164 

 Methyl red reaction, media for, 56 

 Methylene blue, in dilute alcohol, 13 



as indicator of anaerobiosis, 122 

 Microaerophiles, 38 

 Microbiological assay, 192, 195 

 Micromethods for determining biochemi- 

 cal characteristics, 148 

 Microscopic examination of anaerobes, 



129-132 

 Microscopic method, direct, 171 

 Microscopic-slide test for agglutination, 



208 

 Milk, acid production in, 165 



litmus, 56 

 Milk solution for lyophilizing, 100 

 Mineral oil, white, 101 



