314 



MANUAL OF MICROBIOLOGICAL METHODS 



Plants, inoculation methods for, by 

 wounds, 291 



pathogenicity in, 286-298 



treatment of, with bacterial products, 

 293 

 Plating system using strongly reducing 



medium, 128 

 Ponder's diphtheria stain, 24 

 Postinoculation incubation of eggs, 261 

 Postinoculation observation of laboratory 



animals, 254 

 Potato-dextrose agar, 112 

 Potato infusion, 49 

 Potato slants, 57 

 Potato-starch agar, 113 

 Potentiometric method, for oxygen- 

 reduction measurements, 91-94 



for pH measurement, 72-77 

 Practical hints, 7-9 

 Precipitation, 209-214 



m gels, 213 

 Precipitins, quantitative measurement of, 

 214 



ring test for, 210 



supernate test for, 213 

 Preparation, of antigen for agglutination, 

 209 



of inocula, 228-229 



of resting cell suspensions, 175 



of sheep erythrocytes, 215 

 Preservation of cultures, 99-119 

 Products of dissimilation, 183-187 

 Protein antigens, 204 



soluble, 201 

 Protein estimation, 179-181 



by U.V. absorption, 180 

 Protein fractionation, 178 

 Protein metabolism, 190 

 Protocol for plant inoculations, 297 

 "Pure Culture Study of Bacteria, 



Manual of Methods for," 2 

 Purification of antibody, 203 

 Purine metabolism, 193 

 Pyrimidine metabolism, 193 

 Pyrogallol, alkaline, for oxygen removal, 

 126 



Quantitative measurement, of agglutinin, 

 208 

 of precipitin, 214 

 Quinhydrone electrode, 74-75 



Reaction of media, 39 



Reactions involving oxidation reduction, 

 188-190 



Receptor-destroying enzyme, 274 



Recovery of bacteria from infected 

 animals, 235-244 



Reducing medium, plating system using, 

 128 



Relation of organisms to free oxygen, 152 



Rennet production, 165 



Resting cell suspensions, preparation of, 

 175 



Rhizobium medium, 113 



Rickettsiae, 246 

 stain for, 31 

 storing of, 283 



Ring test for precipitins, 210 



Robinov's stain for nuclear apparatus, 30 



Rose bengal, carbol, 13 



Routine tests for identification of bac- 

 teria, 140-168 



Seed inoculation of plants, 289 

 Semisolid agar, 45, 53 



NIH, 111 

 Sensitization of erythrocytes, 216 

 Serological methods, 199-223 



for viruses, 271-280 

 Serological reactions of anaerobes, 137 

 Serum, coagulated, 55 

 Serum-dilution method for precipitation 



test, 210 

 Sheep erythrocytes, preparation of, 215 

 Significance of Eh measurements, 93 

 Silica gel, 45 



Slide test for agglutination, macroscopic, 

 208 



microscopic, 208 

 Smears, preparation of, 11 

 Sodium caseinate agar, 43 

 Soil cultures, 104 

 Soil inoculation, 289 

 Solubilities, dye, 32-34 

 Soluble protein antigens, 201 

 Soybean meal, papaic digest of, 69 

 Specifications, for agar, 39, 64-67 



for gelatin, 39, 67-69 



for peptone, 39, 69-70 

 Spectrophotometric measurements, 190 

 (SpmZZwm medium, 113 

 Spirochaetes, stains for, 31 



