INDEX 



315 



Spore-staining methods, 20-21 



Bartholomew and Mittwer's "cold," 21 



Dorner's, 20-21 



Wirtz, Conklin's modification of, 21 

 Spores of anaerobes, demonstration of, 



131 

 Sporovibrio medium, 114 

 Spray inoculation of plants, 289 

 Spray's anaerobic dish, 126 

 Staining methods, 10-36 

 Stains, for Negri bodies, 249 



for Rickettsiae, 31 



for viruses, 248 

 Standard descriptive chart, 140-142, 145 

 Standards for peptones and gelatin, 39 

 Staphylococci isolation, medium for, 51 

 Starch, hydrolysis of, 162-163 

 Starch agar, 55 

 Sterilization, 39-40 

 Storage and cultivation media, 41-46 

 Storing of viruses and Rickettsiae, 283 

 Strain variations, 294 

 Study of obligately anaerobic bacteria, 



120-139 

 Subcutaneous inoculation, 231 

 Sugar media, 53-55, 160 

 Sugars, cleavage of, 159-162 

 Sulfur-oxidizing bacteria, media for, 47 

 Supernate test for precipitins, 213 

 Synthetic media, 38, 54 



Taxonomy, bacterial, 1-2 



Technics for study of anaerobic bacteria, 

 129-137 



Test for indole, 156 



Thermal death point, 146 



Thermal death time, 146 



Thiobacillus, 114 



Thioglycollate broth, 43 



Thunberg technic, 188 



Thymol blue, 40, 78-79 



Tissue, harvesting of virus in, 257 



Tissue cultures of viruses, 267-269 



Titratable acidity, 87-90 



Tomato-juice agar, 115 



Tomato-yeast-milk medium, 115 



Toxins, 200 



Toxoids, 200 



Treatment of plants with bacterial prod- 

 ucts, 293 



Triple sugar agar, 54 



Trypticase soy agar, 51 

 Tryptone-glucose-yeast agar, 115 

 Tryptose agar, 51 

 Tube agglutination tests, 207 

 Tunnicliff's stain for Spirochaetes, 31 

 Turbidity, determination of, 173 

 Type Culture Collection, American, 99 



Urea agar, 115 



U.V. absorption, protein estimation by, 

 180 



V-8 juice agar, 115 

 Variation, 147 



phase, 6 



strain, 294 

 Vaspar, 101 



Vegetable-tissue jar, 123 

 Virological methods, 246-285 

 Virulence, factors underlying, 225-228 

 Viruses, 246-285 



complement-fixation test for, 275-280 



concentrating, 280-283 



cultivation of (see Cultivation of 

 viruses) 



filtration of, 251 



inoculation with, 253 

 of plants, 292 



neutralization tests for, 278 



serological methods for, 271-280 



stains for, 248 



storing of, 283 



tissue cultures of, 267-269 

 Voges-Proskauer test, 163-164 



media for, 56 



Weight, dry, of cells, 172 



White mineral oil, 101 



Windowing and inoculation of eggs, 



258-261 

 Wirtz method for spore staining, 



Conklin's modification of, 21 

 Wound inoculation of plants, 291 



Yeast-extract-mannitol agar, 46 

 Yeast infusion, 44 

 Yeast-infusion-glucose agar, 46 

 Yolk fluid, harvesting, 263 

 Yolk sac in virus cultivation, 259 



Ziehl's carbol-fuchsin, 13 



