Ferrihaemoprotein Hydroxides 



123 



600 to 650 mil, and the values are also increased a little in the region 480 to 

 510 m/v. Further calculations using //; = 2-84 B.M. gave almost identical 

 spectra; the absorption bands occur at the same wavelengths, and the 

 extinction coefficients change by only about 7 % (see lines d and e of Table 4). 



Table 4. Calculated band maxima and millimolar extinction coefficients 



(given in brackets) for the high- and low-spin hydroxides in the 



wavelength range 480 to 650 m/< 



/'Mb = 5-11: /(Hb = 4-47: //per = 2-66 



• Band at about 490 in/« not fully resolved. 



Abbreviations: Hb, haemoglobin; Mb, myoglobin; Per, peroxidase; 



Leg Hb, leghaemoglobin. 



The absorption curves for the hydroxides of Japanese root peroxidase 

 and leghaemoglobin can be utilized in similar calculations, although at 

 present assumptions have to be made as to the values of their magnetic 

 moments. Adopting 2-66 B.M. for the Japanese root peroxidase derivative, 

 like that for horseradish peroxidase, apg^, the fraction of the low-spin form. 



■'mM 



650 600 550 



A(nnjj) 



500 



Fig. 14. Visible spectra for the high and low-spin hydroxides calculated from 

 the data for ferrimyoglobin and Japanese root ferriperoxidase hydroxide (Morita 



and Kameda, 1958). 



H.E. — VOL. I — K 



