Table 1 



EflFect of dilution of rat erythrocyte haemolysates on 

 the irreversible inhibition of catalase by 3-amino- 

 1 :2:4-triazole. Final concentrations: 0-02 m AT; 

 2 X lO^^Mascorbate; 002 m NaHCOg buffer pH 8-5 

 and varying concentrations of rat erythrocyte haemo- 

 lysate calculated as a dilution from the original 

 volume of packed erythrocytes. Incubated at 37 C for 

 2 hr in air. At the end of the incubation, samples 

 were suitably diluted and catalatic activity determined 

 by the method of Feinstein (1949). 



lOOr- 



5 10 15 



DIALYSIS TIME (HOURS) 

 Fig. 1 . Effect of dialysis on the irreversible inhibition of catalase by 3-amino- 

 1 :2:4-triazole in rat blood haemolysates. A 1 :5 rat erythrocyte haemolysate was 

 dialysed in cellophane tubing against running tap water at room temperature. A 

 control haemolysate was kept at the same temperature. At intervals samples were 

 removed and incubated with : 0-02 m AT; 2 x lO"'* m ascorbate; 0-02 m NaHCOj 

 buffer at pH 8-5 for 2 hr at 37°C in air. Final concentration of haemolysate in 

 incubation mixture 1:7. At the end of incubation samples were removed, suitably 

 diluted and catalase activity determined. 



