270 



LuciLE Smith and Helen Conrad 



Table 3. Guinea pig kidney mitochondria 



A 



4 //I. of mitochondrial suspension in 0-25 M sucrose was added to 



2-6 ml of buffer, then cytochrome c added at times indicated to a 



final concn. of 8 ^m. 



B 



3 ftl. of guinea pig kidney mitochondria was added to test mixture at time, and the 

 optical density readings for the first 20 sec were discarded (see text). 8 /ul. of heart 

 muscle particles was used in the test. The sucrose-KCl medium contained 1 7 1 g sucroses, 

 0-30 g KCl and 0-121 g MgCl^ in 100 ml 002 m phosphate buffer pH 7-4 



Cytochrome c oxidase 

 1st order velocity k x dilution 



in sucrose-KCl 

 medium 



in 0-05 M 

 phosphate buflfer 



Guinea pig kidney mitochondria 

 Heart muscle particles 



60 

 7-0 



30-0 

 8-3 



Table 4. Cytochrome c oxidase of rat heart mitochondria 



The concentration of cytochrome c in the test mixture was 5-1 ftM. The sucrose-versene mixture 

 was 0-32 M sucrose, 0-001 M versene, 002 m phosphate, 0-01 m KCl, pH 7-4 



