CHAPTER 6 



Cultivation and Identification 

 of Bacteria 



INTRODUCTION 



PURE CULTURE ISOLATION TECHNICS 

 Broth dilution 

 Agar dilution 

 Streak plates 



Selective and enrichment media 

 Micromanipulators for single cell isolation 

 Selective heating 

 Use of laboratory animals 



IDENTIFICATION OF PURE CULTURES 



Morphology 



Cultural characteristics 



Physiology 



Serology 



INTRODUCTION 



Because microbiology is an entirely new field to most persons en- 

 rolled in a survey course of this type, the student must be intro- 

 duced to a new language. With a little effort, a student can readily 

 expand his vocabulary by several hundred scientific words, and if 

 he has studied Latin or has been exposed to some Greek, many 

 terms used in microbiology will be familiar to him. 



An agreement as to the meaning of common terms seems ap- 

 propriate at the outset of this chapter. When bacteria are trans- 

 ferred from one medium to another, the material being transferred 

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