Cultivation and Identification of Bacteria 



127 



DESCRIPTIVE CHART FOR BACTERIA 



I. Morphology 

 VEGETATIVE CELLS: 



Gram staining reaction: 



Form: spheres, short rods, long rods, 

 filaments, spirals. 



Size : Sketch : 



Motility: present, absent. 

 Arrangement: singles, pairs, 



chains, tetrads, clusters, cubical 

 packets. 

 Spores: present, absent. 



Location: central, terminal, 



subterminal. 

 Sporangia: swollen, not swollen. 



II. Cultural Characteristics 



COLONY: Medium Age: 



Form: punctiform, circular, rhizoid, 



irregular. 

 Surface: smooth, rough, dry, moist, 



dull, glistening. 

 Elevation : flat, raised. 

 Edge: entire, wavy, filamentous. 

 Growth: slow, moderate, rapid. 



SLANT: Medium: Age: 



Form: thread-like, beaded, root-like, 



spreading. 

 Consistency: butyrous, viscid, brittle. 

 Medium: grayed, browned, greened, 



unchanged. 

 Optical characters: translucent, 



opaque, irridescent. 

 Color: water soluble, 



water insoluble. 



NUTRIENT BROTH: Age: 



Surface growth: ring, pellicle, none. 



Clouding: slight, moderate, heavy, none. 



Amount of sediment: none, scanty, 

 abundant. 



Type of Sediment : flaky, granular, vis- 

 cid on agitation. 



EN DO OR EOS IN METHYLENE 

 BLUE AGAR: 



Growth: present, absent. 

 Color: present, absent. 

 Metallic sheen: present, absent. 



III. Physiology 

 FERMENT A TIONS: 



Glucose : acid, gas, negative. 

 Age: 



Lactose: acid, gas, negative. 

 Age: 



BROM C RE SOL PURPLE MILK: 



Reaction: acid, neutral, or alkaline. 



Age : 



Curds: acid, rennet, none. 

 Proteolysis: none, slight, moderate, 

 complete. 

 Age : 



METHYLENE BLUE MILK: 

 Reduction: none, slight, moderate, 

 complete. 



Age : 



GELATIN LIQUEFACTION: 

 None, moderate, complete. 

 Slow, moderate, rapid. 



INDOLE TEST: positive, negative. 



METHYL RED TEST: positive, 

 negative. 



VOGES-PROSKAUER TEST: posi- 

 tive, negative. 



CITRATE TEST: positive, negative. 



UREA TEST: positive, negative. 



IV. Additional Data 



Name of organism concluded from the 

 above reactions: 



Student: 

 Dates of study: 



