70 



Essays in Biochemistry 



both backbone and side chain were kept constant with respect to size 

 but varied in ratio, it was found that doubling the ratio of side chain 

 per azide increased the sedimentation rate of the conjugate from 1.2 

 to 1.4 S units. The sedimentation rate of the backbone peptide used 

 in this experiment was 1.0 S units. 



00 2 4 6 



48 



72 



10 12 14 16 18 20 22 



Hours 



Time 



Fig. 2. 1 liter of 2.6% conjugate infused in 1 hr. 



- 6«" 



- 2 P 



JS 



CL, 



7 days 



The experience gained in the work described above has led to the 

 production of many preparations which, when clinically tested, have 

 given satisfactory results with respect to blood-stream retention, 

 plasma volume extension, and absence of unfavorable physiologic or 

 hematologic effects. A typical example is shown in Fig. 2 and Table 2. 



Table 2. Clinical Test: Hematological Findings 



(1 liter of 2.6% conjugate infused in 1 hour) 



