284 



Essays in Biochemistry 



Effluent liters 1.0 



•Gradually increasing pH, 3.1 



2.0 



4.3, at constant [Na + ], 35° 



,2? 10 



[Cys 2 , Asp 2 (xNH 2 ), Glu 3 CyNH 2 ), Ala,, Val 4 , Leu, Pro, 

 Phe, Ser 2 , Thr, His, Lys]Lys (30; 50%)\ ft 9 



0.5 



Peptide 9 



Effluent liters 2.0 3.0 



— | Gradually increasing pH and [Na + ], (0.2 N pH 3.1— 0.5 N pH 5.1), 50° |— 



-3 1.0 



0.5 - 



Effluent liters 2.0 3.0 



— | Gradually increasing pH and [Na + ], (0.2 N pH 3.1— 1.0 iST pH 5.1), 50° |— 



Fig. 4. Rechromatography of peptides from a tryptic hydrolyzate of oxidized 

 ribonuclease on a 150 X 2 cm. column of Dowex 50-X2. The peptides fraction- 

 ated are taken from the overlapping peaks shown in Fig. 3, except that peptides 

 2, 3, and 4 were obtained from a 3-hour tryptic hydrolyzate. 



