APPENDIX 419 



structures in the nucleus, and also protoplasmic projections. 



Neutral red (1:3,000 to 30,000) to stain the nucleus and 

 to test the alkalinity (yellowish red) or acidity (cherry red) of 

 food vacuoles. 



Parasitic Protozoa should be studied in the tissue or body 

 fluid of the host animals in which they occur. When these are 

 unavailable or too small in amount to make a suitable prepara- 

 tion, one of the following solutions may be substituted: 



Physiological salt solution. The standard concentration of 

 the solution of sodium chloride is 0.7 per cent for cold-blooded 

 animals and 0.85 per cent for warm-blooded animals. 



Ringer's solution. Various modifications have been pro- 

 posed. The commonly used one consists of the following: 



Sodium chloride 0.8 gm. 



Potassium chloride 0.02 gm. 



Calcium chloride 0.02 gm. 



Sodium bicarbonate 0.02 gm. 



Distilled water 100 c.c. 



References 



Belar, K. 1928 Untersuchung der Protozoen. Methodik der 



wiss. Biologie. Vol. 1. 

 DoFLEiN, F. AND E. Reichenow. 1929 Lehrbuch der Proto- 



zoenkunde. Jena. 

 Gatenby, J. B. AND E. V. CowDRY. 1928 Bolles Lee's micro- 



tomist's vade mecum. London. 

 Hegner, R. and J. Andrews. 1930 Problems and methods of 



research in protozoology. New York. 

 McClung, C. E. 1929 Handbook of microscopical technique. 



New York. 



