468 D. W. BRUNER AND P. R. EDWARDS 



phases identical with that of Salmonella choUrae-suis from cul- 

 tures of S. cholerae-suis var. kunzendorf. Bruner and Edwards 

 (1939) isolated specific phases from Salmonella typhi-murium var. 

 binns, Salmonella typhi-suis var. voldagsen, S. cholerae-suis var. 

 kunzendorf and the Berlin and Puerto Rico types by cultivation 

 in immune serums. In each instance the specific phases so iso- 

 lated were identical with those that occur naturally in the diphasic 

 counterparts of the variants. Edwards and Bruner (1939a) work- 

 ing with the naturally monophasic Salmonella abortus-equi iso- 

 lated two additional phases by cultivation of the organism in 

 various immune serums. One of these was an "artificial" phase 

 closely related to the induced phase of the Schleissheim type 

 described by Kauffman and Tesdal. The second induced phase, 

 on the contrary, was the antigen a of the Kauffmann-White 

 classification and was identical with the flocculating antigen of 

 Salmonella paratyphi A. 



The purpose of the present work is to describe the isolation of 

 phases closely related to the naturally occurring non-specific 

 phases of the genus Salmonella from S. paratyphi A. It is realized 

 that the serology of the Salmonellas is already sufficiently com- 

 plicated and that work such as that presented here may seem on 

 first thought only to confuse the classification of the bacilli. 

 However, the demonstration of inapparent components of the 

 bacilli adds to our knowledge of the genetic relationships and 

 hereditary tendencies in the genus. 



MATERIALS AND METHODS 



Sixteen cultures of S. paratyphi A were used in the study. 

 The sources and designations of the strains were as follows: 



1015, Marta, HA1, HA6, Durazzo— From Dr. F. Kauffmann, 

 International Salmonella Center, Copenhagen. 



38250, 37407— From Dr. Ruth Gilbert, New York State Depart- 

 ment of Health. 



Fried, Boyd, 17, W, WB39— From Dr. Ralph Muckenfuss, New 

 York City Department of Health. 



228, HR, GV— From Dr. L. F. Rettger, Yale University. 



Cal. 49— From Dr. W. R. Hinshaw. Stock culture from Medical 

 School, University of California. 



105 



